Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

A novel nitroreductase-enhanced MRI contrast agent and its potential application in bacterial imaging

[thumbnail of Article]
Preview
PDF (Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
1MB
[thumbnail of Supplementary Material]
Preview
PDF (Supplementary Material) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
977kB

Item Type:Article
Title:A novel nitroreductase-enhanced MRI contrast agent and its potential application in bacterial imaging
Creators Name:Liu, Y., Zhang, L., Nazare, M., Yao, Q. and Hu, H.Y.
Abstract:Nitroreductases (NTRs) are known to be able to metabolize nitro-substituted compounds in the presence of reduced nicotinamide adenine dinucleotide (NADH) as an electron donor. NTRs are present in a wide range of bacterial genera and, to a lesser extent, in eukaryotes hypoxic tumour cells and tumorous tissues, which makes it an appropriate biomarker for an imaging target to detect the hypoxic status of cancer cells and potential bacterial infections. To evaluate the specific activation level of NTR, great efforts have been devoted to the development of fluorescent probes to detect NTR activities using fluorogenic methods to probe its behaviour in a cellular context; however, NTR-responsive MRI contrast agents are still by far underexplored. In this study, para-nitrobenzyl substituted T1-weighted magnetic resonance imaging (MRI) contrast agent Gd-DOTA-PNB (probe 1) has been designed and explored for the possible detection of NTR. Our experimental results show that probe 1 could serve as an MRI-enhanced contrast agent for monitoring NTR activity. The in vitro response and mechanism of the NTR catalysed reduction of probe 1 have been investigated through LC-MS and MRI. Para-nitrobenzyl substituted probe 1 was catalytically reduced by NTR to the intermediate para-aminobenzyl substituted probe which then underwent a rearrangement elimination reaction to Gd-DOTA, generating the enhanced T1-weighted MR imaging. Further, LC-MS and MRI studies of living Escherichia coli have confirmed the NTR activity detection ability of probe 1 at a cellular level. This method may potentially be used for the diagnosis of bacterial infections.
Keywords:Nitroreductase, MRI Contrast Agent, Smart Imaging Probes, Bacterial Imaging, Bacterial Infection
Source:Acta Pharmaceutica Sinica B
ISSN:2211-3835
Publisher:Elsevier
Volume:8
Number:3
Page Range:401-408
Date:May 2018
Official Publication:https://doi.org/10.1016/j.apsb.2017.11.001
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library