%PDF-1.6
%
1 0 obj
<>
endobj
2 0 obj
<>stream
Jeanie K. Meckes, Patrick H. Lim, Stephanie L. Wert, Wendy Luo, Stephanie A. Gacek, Dana Platt, Ryan Jankord, Kathrin Saar, Eva E. Redei
Brain region-specific expression of genes mapped within quantitative trait loci for behavioral responsiveness to acute stress in Fisher 344 and Wistar Kyoto male rats
www.plosone.org
www.plosone.org
endstream
endobj
3 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 15 0 R/Annots 16 0 R/Contents 17 0 R/TrimBox[0 0 612 792]>>
endobj
16 0 obj
[18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R 27 0 R 28 0 R 29 0 R 30 0 R 31 0 R 32 0 R 33 0 R 34 0 R 35 0 R 36 0 R 37 0 R 38 0 R 39 0 R 40 0 R 41 0 R 42 0 R]
endobj
18 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref001)>>
endobj
19 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref003)>>
endobj
20 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref004)>>
endobj
21 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref007)>>
endobj
22 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref008)>>
endobj
23 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref009)>>
endobj
24 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref010)>>
endobj
25 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref014)>>
endobj
26 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref015)>>
endobj
27 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref019)>>
endobj
28 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref019)>>
endobj
29 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.t001)>>
endobj
30 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref016)>>
endobj
31 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref019)>>
endobj
32 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref019)>>
endobj
33 0 obj
<>/Border[0 0 0]/A 43 0 R>>
endobj
43 0 obj
<>
endobj
34 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref012)>>
endobj
35 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref014)>>
endobj
36 0 obj
<>/Border[0 0 0]/Dest(Lpone.0194293.t001)>>
endobj
37 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref015)>>
endobj
38 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref019)>>
endobj
39 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref019)>>
endobj
40 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref019)>>
endobj
41 0 obj
<>/Border[0 0 0]/A 44 0 R>>
endobj
44 0 obj
<>
endobj
42 0 obj
<>/Border[0 0 0]/A 45 0 R>>
endobj
45 0 obj
<>
endobj
17 0 obj
[46 0 R 47 0 R 48 0 R 49 0 R 50 0 R 51 0 R 52 0 R 53 0 R 54 0 R 55 0 R 56 0 R 57 0 R 58 0 R 59 0 R 60 0 R 61 0 R]
endobj
46 0 obj
<>stream
q
0.83 0.64 0.02 0 k
251.8866 649.0205 m
256.6488 649.0205 l
h
f*
261.8079 649.0205 m
266.5701 649.0205 l
h
f*
373.4929 635.9811 m
378.2551 635.9811 l
h
f*
383.4142 635.9811 m
388.1764 635.9811 l
h
f*
419.9244 622.9984 m
424.6866 622.9984 l
h
f*
429.1654 622.9984 m
433.9276 622.9984 l
h
f*
294.1795 596.9764 m
303.7039 596.9764 l
h
f*
308.863 596.9764 m
318.3874 596.9764 l
h
f*
443.5654 544.989 m
453.0331 544.989 l
h
f*
458.2488 544.989 m
467.7165 544.989 l
h
f*
540.0567 532.0063 m
549.5244 532.0063 l
h
f*
484.3276 466.9795 m
513.4677 466.9795 l
h
f*
522.4819 466.9795 m
532.0063 466.9795 l
h
f*
458.6457 428.0315 m
468.1701 428.0315 l
h
f*
514.9417 402.0094 m
524.4661 402.0094 l
h
f*
241.1717 272.0126 m
314.9291 272.0126 l
h
f*
533.0268 272.0126 m
542.3811 272.0126 l
h
f*
546.8031 272.0126 m
556.1575 272.0126 l
h
f*
0 g
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 706.0535 cm
BT
/F2 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Introduction)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 689.1023 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Acute)Tj
2.5738 0 Td
(stress)Tj
2.3981 0 Td
(is)Tj
0.8504 0 Td
(a)Tj
0.6519 0 Td
(common)Tj
3.8438 0 Td
(occurrence)Tj
4.6658 0 Td
(in)Tj
1.0318 0 Td
(everybody's)Tj
4.9209 0 Td
(life)Tj
1.4457 0 Td
(with)Tj
1.9955 0 Td
(differing)Tj
3.6681 0 Td
(severity.)Tj
3.4979 0 Td
(Individual)Tj
4.3653 0 Td
(var-)Tj
-35.909 -1.3039 Td
(iation)Tj
2.5398 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(response)Tj
3.736 0 Td
(to)Tj
1.0205 0 Td
(acute)Tj
2.3301 0 Td
(stress)Tj
2.3924 0 Td
(depends)Tj
3.5546 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(sensitivity)Tj
4.218 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4683 0 Td
(stressor)Tj
3.2769 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4683 0 Td
(ability)Tj
2.6986 0 Td
(to)Tj
-36.7084 -1.2982 Td
(cope)Tj
2.0919 0 Td
(with)Tj
1.9956 0 Td
(it.)Tj
1.0091 0 Td
(Genetic)Tj
3.3222 0 Td
(studies)Tj
2.9877 0 Td
(support)Tj
3.3165 0 Td
(the)Tj
1.4683 0 Td
(assumption)Tj
4.8643 0 Td
(that)Tj
1.7801 0 Td
(acute)Tj
2.3244 0 Td
(stress)Tj
2.3981 0 Td
(responsiveness)Tj
6.1455 0 Td
(is)Tj
0.8504 0 Td
(a)Tj
0.6519 0 Td
(quan-)Tj
-35.206 -1.2983 Td
(titative)Tj
2.9593 0 Td
(trait)Tj
1.8879 0 Td
([)Tj
0.83 0.64 0.02 0 k
(1)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(3)Tj
0 g
(].)Tj
2.5965 0 Td
(Its)Tj
1.2189 0 Td
(narrow-sense)Tj
5.6069 0 Td
(heritability)Tj
4.5807 0 Td
(ranges)Tj
2.8233 0 Td
(between)Tj
3.5263 0 Td
(0.40.6)Tj
3.0897 0 Td
(defined)Tj
3.2258 0 Td
(by)Tj
1.1849 0 Td
(multivariate)Tj
-32.7002 -1.3039 Td
(genetic)Tj
3.0613 0 Td
(analyses)Tj
3.4753 0 Td
(in)Tj
1.0318 0 Td
(family)Tj
2.7212 0 Td
(and)Tj
1.7235 0 Td
(twin)Tj
2.0126 0 Td
(studies)Tj
2.982 0 Td
([)Tj
0.83 0.64 0.02 0 k
(4)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(].)Tj
2.5852 0 Td
(The)Tj
1.7801 0 Td
(heritability)Tj
4.5751 0 Td
(of)Tj
1.0092 0 Td
(acute)Tj
2.3187 0 Td
(stress)Tj
2.3924 0 Td
(responsiveness)Tj
-31.6684 -1.2983 Td
(is)Tj
0.8447 0 Td
(estimated)Tj
4.0762 0 Td
(to)Tj
1.0261 0 Td
(be)Tj
1.1509 0 Td
(similar)Tj
2.982 0 Td
(in)Tj
1.0375 0 Td
(rodents)Tj
3.2598 0 Td
(to)Tj
1.0204 0 Td
(that)Tj
1.7802 0 Td
(in)Tj
1.0375 0 Td
(humans)Tj
3.4355 0 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.2643 0 Td
(9)Tj
0 g
(].)Tj
1.2586 0 Td
(However,)Tj
4.0762 0 Td
(only)Tj
1.9785 0 Td
(a)Tj
0.652 0 Td
(limited)Tj
3.0727 0 Td
(number)Tj
-33.9531 -1.2982 Td
(of)Tj
1.0147 0 Td
(candidate)Tj
4.1046 0 Td
(genes)Tj
2.4378 0 Td
(have)Tj
2.0636 0 Td
(been)Tj
2.1203 0 Td
(proposed)Tj
3.9514 0 Td
(for)Tj
1.389 0 Td
(individual)Tj
4.286 0 Td
(variation)Tj
3.804 0 Td
(in)Tj
1.0318 0 Td
(behavioral)Tj
4.3937 0 Td
(acute)Tj
2.3301 0 Td
(stress)Tj
-32.927 -1.304 Td
(responsiveness)Tj
6.1454 0 Td
(to)Tj
1.0262 0 Td
(date)Tj
1.9048 0 Td
([)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(].)Tj
3.5433 0 Td
(The)Tj
1.7858 0 Td
(purpose)Tj
3.4413 0 Td
(of)Tj
1.0148 0 Td
(this)Tj
1.6781 0 Td
(study)Tj
2.3867 0 Td
(was)Tj
1.7008 0 Td
(to)Tj
1.0261 0 Td
(identify)Tj
3.2939 0 Td
(candidate)Tj
4.1045 0 Td
(genes)Tj
2.4435 0 Td
(with)Tj
-35.4952 -1.2982 Td
(sequence)Tj
3.855 0 Td
(variations)Tj
4.1669 0 Td
(that)Tj
1.7745 0 Td
(contribute)Tj
4.3937 0 Td
(to)Tj
1.0261 0 Td
(variations)Tj
4.1613 0 Td
(in)Tj
1.0374 0 Td
(individual)Tj
4.286 0 Td
(stress)Tj
2.3981 0 Td
(vulnerability.)Tj
-25.9028 -1.2983 Td
(We)Tj
1.61 0 Td
(have)Tj
2.0636 0 Td
(studied)Tj
3.1521 0 Td
(the)Tj
1.4684 0 Td
(genetic)Tj
3.0727 0 Td
(components)Tj
5.1704 0 Td
(of)Tj
1.0148 0 Td
(behavioral)Tj
4.3937 0 Td
(responsiveness)Tj
6.1511 0 Td
(to)Tj
1.0205 0 Td
(acute)Tj
2.33 0 Td
(stress)Tj
2.3981 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
-36.0734 -1.2983 Td
(reciprocally)Tj
4.9039 0 Td
(crossed)Tj
3.1974 0 Td
(F)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 286.2992 557.4047 cm
BT
/F6 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 292.0251 559.1055 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(generation)Tj
4.507 0 Td
(of)Tj
1.0148 0 Td
(Wistar)Tj
2.9197 0 Td
(Kyoto)Tj
2.6589 0 Td
(\(WKY\))Tj
3.1747 0 Td
(and)Tj
1.7292 0 Td
(Fischer)Tj
3.118 0 Td
(344)Tj
1.6498 0 Td
(\(F344\))Tj
2.8516 0 Td
(rat)Tj
1.3266 0 Td
(strains)Tj
-34.1515 -1.3039 Td
(using)Tj
2.3868 0 Td
(the)Tj
1.4683 0 Td
(defensive)Tj
3.9345 0 Td
(burying)Tj
3.3561 0 Td
(\(DB\))Tj
2.2167 0 Td
(and)Tj
1.7234 0 Td
(open)Tj
2.2167 0 Td
(field)Tj
1.9672 0 Td
(tests)Tj
1.9616 0 Td
(\(OFT\))Tj
2.7836 0 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(].)Tj
3.5433 0 Td
(The)Tj
1.7745 0 Td
(parental)Tj
3.4922 0 Td
(WKY)Tj
2.4888 0 Td
(strain)Tj
-35.3137 -1.2982 Td
(consistently)Tj
4.955 0 Td
(exhibits)Tj
3.3392 0 Td
(hypoactive)Tj
4.5296 0 Td
(and)Tj
1.7291 0 Td
(avoidant)Tj
3.6907 0 Td
(behavior)Tj
3.7134 0 Td
(compared)Tj
4.2406 0 Td
(to)Tj
1.0261 0 Td
(that)Tj
1.7802 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(F344)Tj
2.177 0 Td
([)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(].)Tj
2.0749 0 Td
(We)Tj
-35.7389 -1.2983 Td
(argue)Tj
2.4378 0 Td
(that)Tj
1.7802 0 Td
(this)Tj
1.6724 0 Td
(consistent)Tj
4.2575 0 Td
(repertoire)Tj
4.1953 0 Td
(of)Tj
1.0148 0 Td
(behavior)Tj
3.7133 0 Td
(across)Tj
2.6759 0 Td
(various)Tj
3.1464 0 Td
(tests)Tj
1.9616 0 Td
(is)Tj
0.8447 0 Td
(not)Tj
1.5761 0 Td
(composed)Tj
4.3029 0 Td
(of)Tj
1.0148 0 Td
(dis-)Tj
-34.5937 -1.3039 Td
(crete,)Tj
2.3868 0 Td
(individual)Tj
4.286 0 Td
(reactions)Tj
3.855 0 Td
(to)Tj
1.0261 0 Td
(each)Tj
2.0239 0 Td
(situation,)Tj
3.9572 0 Td
(but)Tj
1.5533 0 Td
(belies)Tj
2.4492 0 Td
(a)Tj
0.6519 0 Td
(more)Tj
2.3414 0 Td
(global)Tj
2.6249 0 Td
(response)Tj
3.736 0 Td
(to)Tj
1.0205 0 Td
(an)Tj
1.2075 0 Td
(acute)Tj
2.3244 0 Td
(chal-)Tj
-35.4441 -1.2983 Td
(lenge)Tj
2.3244 0 Td
(with)Tj
2.0013 0 Td
(substantial)Tj
4.5014 0 Td
(genetic)Tj
3.0669 0 Td
(origin.)Tj
2.8744 0 Td
(Among)Tj
3.2428 0 Td
(the)Tj
1.4683 0 Td
(quantitative)Tj
4.9833 0 Td
(trait)Tj
1.8878 0 Td
(loci)Tj
1.6611 0 Td
(\(QTL\))Tj
2.795 0 Td
(found)Tj
2.6192 0 Td
(in)Tj
1.0375 0 Td
(these)Tj
-34.4634 -1.2982 Td
(studies,)Tj
3.2088 0 Td
(a)Tj
0.6577 0 Td
(highly)Tj
2.7042 0 Td
(significant)Tj
4.3879 0 Td
(locus)Tj
2.2904 0 Td
(on)Tj
1.2756 0 Td
(chromosome)Tj
5.4822 0 Td
(6,)Tj
/F10 1 Tf
0.924 0 Td
[()10()7()20()8()10()]TJ
/F6 1 Tf
(,)Tj
3.6851 0 Td
(has)Tj
1.542 0 Td
(been)Tj
2.126 0 Td
(identified)Tj
4.0592 0 Td
(for)Tj
1.3832 0 Td
(multiple)Tj
-33.7263 -1.304 Td
(phenotypes;)Tj
5.023 0 Td
(a)Tj
0.652 0 Td
(potential)Tj
3.7643 0 Td
(pleiotropy)Tj
4.3256 0 Td
(for)Tj
1.389 0 Td
(behavioral)Tj
4.3936 0 Td
(stress)Tj
2.3925 0 Td
(responsiveness)Tj
6.1511 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Table)Tj
2.7836 0 Td
(1)Tj
0 g
(\))Tj
1.0318 0 Td
([)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(].)Tj
2.0806 0 Td
(The)Tj
1.7802 0 Td
(phe-)Tj
-35.7673 -1.2982 Td
(notypes)Tj
3.3279 0 Td
(associated)Tj
4.2576 0 Td
(with)Tj
1.9955 0 Td
(this)Tj
1.6781 0 Td
(QTL)Tj
2.1146 0 Td
(include)Tj
3.1861 0 Td
(latency)Tj
3.0444 0 Td
(to)Tj
1.0262 0 Td
(bury,)Tj
2.3074 0 Td
(grooming,)Tj
4.3993 0 Td
(rearing)Tj
3.1011 0 Td
(in)Tj
1.0318 0 Td
(DB,)Tj
1.7631 0 Td
(and)Tj
1.7291 0 Td
(rear-)Tj
-34.9622 -1.2983 Td
(ing)Tj
1.5024 0 Td
(in)Tj
1.0318 0 Td
(OFT.)Tj
2.3301 0 Td
(It)Tj
0.8617 0 Td
(is)Tj
0.8447 0 Td
(notable)Tj
3.186 0 Td
(that)Tj
1.7802 0 Td
(a)Tj
0.6519 0 Td
(discrepancy)Tj
4.972 0 Td
(was)Tj
1.7008 0 Td
(described)Tj
4.0535 0 Td
(for)Tj
1.3833 0 Td
(the)Tj
1.4683 0 Td
(OFT)Tj
2.109 0 Td
(and)Tj
1.7291 0 Td
(DB)Tj
1.5364 0 Td
(rearing)Tj
3.1011 0 Td
(QTLs)Tj
-34.2423 -1.2983 Td
(using)Tj
2.3868 0 Td
(genetic)Tj
3.0727 0 Td
(mapping)Tj
3.787 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
1.4684 0 Td
(time)Tj
2.0296 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(original)Tj
3.3279 0 Td
(publication)Tj
4.7621 0 Td
([)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(].)Tj
2.0807 0 Td
(However,)Tj
4.0761 0 Td
(physical)Tj
3.4526 0 Td
(map-)Tj
-35.1323 -1.3039 Td
(ping)Tj
2.0183 0 Td
(using)Tj
2.3868 0 Td
(the)Tj
1.4683 0 Td
(more)Tj
2.3414 0 Td
(recent)Tj
2.7098 0 Td
(rat)Tj
1.3266 0 Td
(genome)Tj
3.4072 0 Td
(version)Tj
3.1521 0 Td
(Rnor_6.0)Tj
3.9402 0 Td
(identified)Tj
4.0592 0 Td
(that)Tj
1.7801 0 Td
(these)Tj
2.2507 0 Td
(QTLs)Tj
2.4775 0 Td
(indeed)Tj
-33.3182 -1.2982 Td
(overlap.)Tj
3.3959 0 Td
(Furthermore,)Tj
5.6183 0 Td
(rearing)Tj
3.1009 0 Td
(measures)Tj
3.9402 0 Td
(highly)Tj
2.7099 0 Td
(correlate)Tj
3.719 0 Td
(between)Tj
3.5263 0 Td
(the)Tj
1.4683 0 Td
(two)Tj
1.7121 0 Td
(tests)Tj
1.9616 0 Td
([)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(].)Tj
2.075 0 Td
(All)Tj
1.4003 0 Td
(of)Tj
-34.6278 -1.2983 Td
(these)Tj
2.2507 0 Td
(behaviors)Tj
4.0762 0 Td
(represent)Tj
3.9571 0 Td
(a)Tj
0.6519 0 Td
(general)Tj
3.1351 0 Td
(stress)Tj
2.3981 0 Td
(response)Tj
3.7304 0 Td
(to)Tj
1.0261 0 Td
(an)Tj
1.2019 0 Td
(acute)Tj
2.3244 0 Td
(stressor,)Tj
3.5036 0 Td
(whether)Tj
3.4753 0 Td
(it)Tj
0.7823 0 Td
(be)Tj
1.1509 0 Td
(the)Tj
1.474 0 Td
(shock)Tj
-35.138 -1.3039 Td
(in)Tj
1.0319 0 Td
(the)Tj
1.474 0 Td
(DB)Tj
1.5363 0 Td
(or)Tj
1.0942 0 Td
(the)Tj
1.474 0 Td
(novelty)Tj
3.152 0 Td
(of)Tj
1.0148 0 Td
(OFT.)Tj
2.3301 0 Td
(Thus,)Tj
2.4718 0 Td
(this)Tj
1.6724 0 Td
(chromosomal)Tj
5.7486 0 Td
(region)Tj
2.8006 0 Td
(is)Tj
0.8504 0 Td
(likely)Tj
2.3528 0 Td
(to)Tj
1.0261 0 Td
(harbor)Tj
2.9367 0 Td
(one)Tj
1.7007 0 Td
(or)Tj
-34.6674 -1.2983 Td
(more)Tj
2.3415 0 Td
(genes)Tj
2.4377 0 Td
(with)Tj
2.0013 0 Td
(sequence)Tj
3.855 0 Td
(variation\(s\))Tj
4.8472 0 Td
(that)Tj
1.7745 0 Td
(contribute)Tj
4.3936 0 Td
(to)Tj
1.0262 0 Td
(phenotypic)Tj
4.7055 0 Td
(variations)Tj
4.1612 0 Td
(in)Tj
1.0375 0 Td
(general)Tj
-32.5812 -1.2982 Td
(stress)Tj
2.3925 0 Td
(responsiveness)Tj
6.1511 0 Td
(to)Tj
1.0261 0 Td
(acute)Tj
2.3243 0 Td
(stressors)Tj
3.6397 0 Td
(between)Tj
3.5263 0 Td
(these)Tj
2.2563 0 Td
(strains.)Tj
-20.12 -1.304 Td
(In)Tj
1.1111 0 Td
(this)Tj
1.6725 0 Td
(study,)Tj
2.6192 0 Td
(we)Tj
1.3266 0 Td
(aimed)Tj
2.6928 0 Td
(to)Tj
1.0204 0 Td
(identify)Tj
3.2995 0 Td
(candidate)Tj
4.1046 0 Td
(quantitative)Tj
4.9776 0 Td
(trait)Tj
1.8878 0 Td
(genes)Tj
2.4435 0 Td
(\(QTG\))Tj
2.9707 0 Td
(that)Tj
1.7801 0 Td
(contribute)Tj
-0.012 Tc
-33.1027 -1.2982 Td
(to)Tj
1.0092 0 Td
(differences)Tj
4.4787 0 Td
(in)Tj
1.0148 0 Td
(acute)Tj
2.2904 0 Td
(stress)Tj
2.3469 0 Td
(responsiveness)Tj
6.0378 0 Td
(between)Tj
3.4696 0 Td
(F344)Tj
2.143 0 Td
(and)Tj
1.7064 0 Td
(WKY)Tj
2.4718 0 Td
(rat)Tj
1.2983 0 Td
(strains)Tj
2.8062 0 Td
(in)Tj
1.0205 0 Td
(the)Tj
/F10 1 Tf
1.4457 0 Td
[()10()7()20()8()10()]TJ
/F6 1 Tf
-33.5393 -1.2983 Td
(QTL.)Tj
2.3074 0 Td
(To)Tj
1.321 0 Td
(aid)Tj
1.4286 0 Td
(in)Tj
1.0205 0 Td
(the)Tj
1.4457 0 Td
(identification)Tj
5.4707 0 Td
(of)Tj
0.9978 0 Td
(QTG\(s\),)Tj
3.5036 0 Td
(first)Tj
1.7688 0 Td
(we)Tj
1.3096 0 Td
(established)Tj
4.507 0 Td
(the)Tj
1.4457 0 Td
(differentially)Tj
5.193 0 Td
(expressed)Tj
-31.7194 -1.3039 Td
(genes)Tj
2.3981 0 Td
(\(DEGs\))Tj
3.2372 0 Td
(between)Tj
3.4696 0 Td
(the)Tj
1.4455 0 Td
(two)Tj
1.6895 0 Td
(parental)Tj
3.4299 0 Td
(strains)Tj
2.8006 0 Td
(within)Tj
2.7666 0 Td
(the)Tj
/F10 1 Tf
1.4456 0 Td
[()10()7()20()8()10()]TJ
/F6 1 Tf
3.4016 0 Td
(QTL)Tj
2.0919 0 Td
(using)Tj
2.3471 0 Td
(genome-wide)Tj
-30.5232 -1.2983 Td
(microarray)Tj
4.5921 0 Td
(analyses)Tj
3.4186 0 Td
(in)Tj
1.0205 0 Td
(the)Tj
1.4455 0 Td
(hippocampus,)Tj
5.7713 0 Td
(amygdala,)Tj
4.1839 0 Td
(and)Tj
1.7065 0 Td
(frontal)Tj
2.8573 0 Td
(cortex.)Tj
2.88 0 Td
(Using)Tj
2.5568 0 Td
(the)Tj
1.4457 0 Td
(Rat)Tj
1.5533 0 Td
(Genome)Tj
-33.4315 -1.2982 Td
(Database)Tj
3.7814 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(www.rgd.mcw.edu)Tj
0 g
(\))Tj
8.2657 0 Td
(and)Tj
1.7065 0 Td
(the)Tj
1.4456 0 Td
(original)Tj
3.2598 0 Td
(sequences)Tj
4.1499 0 Td
(of)Tj
0.9978 0 Td
(the)Tj
1.4457 0 Td
(two)Tj
1.6838 0 Td
(parental)Tj
3.4299 0 Td
(strains)Tj
2.8062 0 Td
([)Tj
0.83 0.64 0.02 0 k
(12)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7065 0 Td
(14)Tj
0 g
(],)Tj
1.7008 0 Td
(we)Tj
-36.3796 -1.2983 Td
(then)Tj
1.9843 0 Td
(determined)Tj
4.7735 0 Td
(nonsynonymous)Tj
6.8087 0 Td
(sequence)Tj
3.7927 0 Td
(variations)Tj
4.0819 0 Td
(in)Tj
1.0204 0 Td
(the)Tj
1.4457 0 Td
(coding)Tj
2.914 0 Td
(regions)Tj
3.1067 0 Td
(of)Tj
0.9978 0 Td
(DEGs)Tj
2.5682 0 Td
(between)Tj
-33.4939 -1.3039 Td
(the)Tj
1.4457 0 Td
(F344)Tj
2.143 0 Td
(and)Tj
1.7064 0 Td
(WKY)Tj
2.4662 0 Td
(strains.)Tj
3.0216 0 Td
(We)Tj
1.593 0 Td
(hypothesized)Tj
5.3745 0 Td
(that)Tj
1.7461 0 Td
(DEGs)Tj
2.5682 0 Td
(that)Tj
1.7461 0 Td
(show)Tj
2.2677 0 Td
(expression)Tj
4.388 0 Td
(differences)Tj
4.4787 0 Td
(paral-)Tj
-34.9452 -1.2983 Td
(lel)Tj
1.1112 0 Td
(to)Tj
1.0091 0 Td
(behavioral)Tj
4.3144 0 Td
(differences)Tj
4.4729 0 Td
(between)Tj
3.4752 0 Td
(the)Tj
1.4457 0 Td
(two)Tj
1.6838 0 Td
(strains)Tj
2.8063 0 Td
(are)Tj
1.4229 0 Td
(candidate)Tj
4.0366 0 Td
(quantitative)Tj
4.8812 0 Td
(trait)Tj
1.8482 0 Td
(genes.)Tj
2.6192 0 Td
(To)Tj
ET
Q
q
0.45 0.36 0.35 0.01 k
1 j
1 J
0 w
36 203.7523 m
576 203.7523 l
576 203.2523 l
36 203.2523 l
f*
108.4935 203.2523 m
108.4935 190.7524 l
107.9935 190.7524 l
107.9935 203.2523 l
f*
107.9935 190.7524 m
36 190.7524 l
36 191.2524 l
107.9935 191.2524 l
f*
189.2897 203.2523 m
189.2897 190.7524 l
188.7897 190.7524 l
188.7897 203.2523 l
f*
188.7897 190.7524 m
108.4935 190.7524 l
108.4935 191.2524 l
188.7897 191.2524 l
f*
277.1271 203.2523 m
277.1271 190.7524 l
276.6271 190.7524 l
276.6271 203.2523 l
f*
276.6271 190.7524 m
189.2897 190.7524 l
189.2897 191.2524 l
276.6271 191.2524 l
f*
326.6904 203.2523 m
326.6904 190.7524 l
326.1903 190.7524 l
326.1903 203.2523 l
f*
326.1903 190.7524 m
277.1271 190.7524 l
277.1271 191.2524 l
326.1903 191.2524 l
f*
433.9987 203.2523 m
433.9987 190.7524 l
433.4987 190.7524 l
433.4987 203.2523 l
f*
433.4987 190.7524 m
326.6904 190.7524 l
326.6904 191.2524 l
433.4987 191.2524 l
f*
510.5718 203.2523 m
510.5718 190.7524 l
510.0718 190.7524 l
510.0718 203.2523 l
f*
510.0718 190.7524 m
433.9987 190.7524 l
433.9987 191.2524 l
510.0718 191.2524 l
f*
575.9994 190.7524 m
510.5718 190.7524 l
510.5718 191.2524 l
575.9994 191.2524 l
f*
108.4935 190.7524 m
108.4935 178.2524 l
107.9935 178.2524 l
107.9935 190.7524 l
f*
107.9935 178.2524 m
36 178.2524 l
36 178.7525 l
107.9935 178.7525 l
f*
189.2897 190.7524 m
189.2897 178.2524 l
188.7897 178.2524 l
188.7897 190.7524 l
f*
188.7897 178.2524 m
108.4935 178.2524 l
108.4935 178.7525 l
188.7897 178.7525 l
f*
277.1271 190.7524 m
277.1271 178.2524 l
276.6271 178.2524 l
276.6271 190.7524 l
f*
276.6271 178.2524 m
189.2897 178.2524 l
189.2897 178.7525 l
276.6271 178.7525 l
f*
326.6904 190.7524 m
326.6904 178.2524 l
326.1903 178.2524 l
326.1903 190.7524 l
f*
326.1903 178.2524 m
277.1271 178.2524 l
277.1271 178.7525 l
326.1903 178.7525 l
f*
433.9987 190.7524 m
433.9987 178.2524 l
433.4987 178.2524 l
433.4987 190.7524 l
f*
433.4987 178.2524 m
326.6904 178.2524 l
326.6904 178.7525 l
433.4987 178.7525 l
f*
510.5718 190.7524 m
510.5718 178.2524 l
510.0718 178.2524 l
510.0718 190.7524 l
f*
510.0718 178.2524 m
433.9987 178.2524 l
433.9987 178.7525 l
510.0718 178.7525 l
f*
575.9994 178.2524 m
510.5718 178.2524 l
510.5718 178.7525 l
575.9994 178.7525 l
f*
108.4935 178.2524 m
108.4935 165.7525 l
107.9935 165.7525 l
107.9935 178.2524 l
f*
107.9935 165.7525 m
36 165.7525 l
36 166.2525 l
107.9935 166.2525 l
f*
189.2897 178.2524 m
189.2897 165.7525 l
188.7897 165.7525 l
188.7897 178.2524 l
f*
188.7897 165.7525 m
108.4935 165.7525 l
108.4935 166.2525 l
188.7897 166.2525 l
f*
277.1271 178.2524 m
277.1271 165.7525 l
276.6271 165.7525 l
276.6271 178.2524 l
f*
276.6271 165.7525 m
189.2897 165.7525 l
189.2897 166.2525 l
276.6271 166.2525 l
f*
326.6904 178.2524 m
326.6904 165.7525 l
326.1903 165.7525 l
326.1903 178.2524 l
f*
326.1903 165.7525 m
277.1271 165.7525 l
277.1271 166.2525 l
326.1903 166.2525 l
f*
433.9987 178.2524 m
433.9987 165.7525 l
433.4987 165.7525 l
433.4987 178.2524 l
f*
433.4987 165.7525 m
326.6904 165.7525 l
326.6904 166.2525 l
433.4987 166.2525 l
f*
510.5718 178.2524 m
510.5718 165.7525 l
510.0718 165.7525 l
510.0718 178.2524 l
f*
510.0718 165.7525 m
433.9987 165.7525 l
433.9987 166.2525 l
510.0718 166.2525 l
f*
575.9994 165.7525 m
510.5718 165.7525 l
510.5718 166.2525 l
575.9994 166.2525 l
f*
108.4935 165.7525 m
108.4935 153.2526 l
107.9935 153.2526 l
107.9935 165.7525 l
f*
107.9935 153.2526 m
36 153.2526 l
36 153.7526 l
107.9935 153.7526 l
f*
189.2897 165.7525 m
189.2897 153.2526 l
188.7897 153.2526 l
188.7897 165.7525 l
f*
188.7897 153.2526 m
108.4935 153.2526 l
108.4935 153.7526 l
188.7897 153.7526 l
f*
277.1271 165.7525 m
277.1271 153.2526 l
276.6271 153.2526 l
276.6271 165.7525 l
f*
276.6271 153.2526 m
189.2897 153.2526 l
189.2897 153.7526 l
276.6271 153.7526 l
f*
326.6904 165.7525 m
326.6904 153.2526 l
326.1903 153.2526 l
326.1903 165.7525 l
f*
326.1903 153.2526 m
277.1271 153.2526 l
277.1271 153.7526 l
326.1903 153.7526 l
f*
433.9987 165.7525 m
433.9987 153.2526 l
433.4987 153.2526 l
433.4987 165.7525 l
f*
433.4987 153.2526 m
326.6904 153.2526 l
326.6904 153.7526 l
433.4987 153.7526 l
f*
510.5718 165.7525 m
510.5718 153.2526 l
510.0718 153.2526 l
510.0718 165.7525 l
f*
510.0718 153.2526 m
433.9987 153.2526 l
433.9987 153.7526 l
510.0718 153.7526 l
f*
575.9994 153.2526 m
510.5718 153.2526 l
510.5718 153.7526 l
575.9994 153.7526 l
f*
108.4935 153.2526 m
108.4935 140.7526 l
107.9935 140.7526 l
107.9935 153.2526 l
f*
107.9935 140.7526 m
36 140.7526 l
36 141.2527 l
107.9935 141.2527 l
f*
189.2897 153.2526 m
189.2897 140.7526 l
188.7897 140.7526 l
188.7897 153.2526 l
f*
188.7897 140.7526 m
108.4935 140.7526 l
108.4935 141.2527 l
188.7897 141.2527 l
f*
277.1271 153.2526 m
277.1271 140.7526 l
276.6271 140.7526 l
276.6271 153.2526 l
f*
276.6271 140.7526 m
189.2897 140.7526 l
189.2897 141.2527 l
276.6271 141.2527 l
f*
326.6904 153.2526 m
326.6904 140.7526 l
326.1903 140.7526 l
326.1903 153.2526 l
f*
326.1903 140.7526 m
277.1271 140.7526 l
277.1271 141.2527 l
326.1903 141.2527 l
f*
433.9987 153.2526 m
433.9987 140.7526 l
433.4987 140.7526 l
433.4987 153.2526 l
f*
433.4987 140.7526 m
326.6904 140.7526 l
326.6904 141.2527 l
433.4987 141.2527 l
f*
510.5718 153.2526 m
510.5718 140.7526 l
510.0718 140.7526 l
510.0718 153.2526 l
f*
510.0718 140.7526 m
433.9987 140.7526 l
433.9987 141.2527 l
510.0718 141.2527 l
f*
575.9994 140.7526 m
510.5718 140.7526 l
510.5718 141.2527 l
575.9994 141.2527 l
f*
575.9994 95.0026 m
36 95.0026 l
36 95.5026 l
575.9994 95.5026 l
f*
0.83 0.64 0.02 0 k
516.3024 180.1701 m
523.9559 180.1701 l
h
f*
516.3024 167.6976 m
523.9559 167.6976 l
h
f*
516.3024 155.1685 m
523.9559 155.1685 l
h
f*
516.3024 142.6961 m
523.9559 142.6961 l
h
f*
36 81.9213 m
186.7465 81.9213 l
h
f*
0 g
7.9999 0 0 7.9999 36 208.5732 cm
BT
/F11 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Table)Tj
2.5512 0 Td
(1.)Tj
1.1763 0 Td
(List)Tj
1.7717 0 Td
(of)Tj
1.063 0 Td
(behavior)Tj
3.6213 0 Td
(al)Tj
0.9567 0 Td
(phenotypes)Tj
4.9678 0 Td
(associated)Tj
4.4362 0 Td
(with)Tj
2.0693 0 Td
(the)Tj
/F12 1 Tf
1.5378 0 Td
[()8()-2()8()-2()8()]TJ
/F11 1 Tf
3.593 0 Td
(QTL.)Tj
0 Tc
-27.7443 -1.8496 Td
(QTL)Tj
2.1826 0 Td
(Symbol)Tj
7.1292 0 Td
(Position)Tj
10.0985 0 Td
(Behavior)Tj
10.9773 0 Td
(LOD)Tj
6.2008 0 Td
(Pointwis)Tj
3.6709 0 Td
(e)Tj
0.6662 0 Td
(significance)Tj
9.078 0 Td
(Varian)Tj
2.8914 0 Td
(ce)Tj
1.0913 0 Td
(\(%\))Tj
5.5843 0 Td
(Reference)Tj
/F10 1 Tf
-59.5705 -1.5661 Td
[()10()7()20()8()10()]TJ
/F6 1 Tf
9.4394 0 Td
(6:135,6)Tj
3.3591 0 Td
(23,029)Tj
6.7394 0 Td
(Latency)Tj
3.3662 0 Td
(\(DB\))Tj
7.6111 0 Td
(3.55)Tj
6.1937 0 Td
(0.00002)Tj
13.4151 0 Td
(3.5)Tj
9.5741 0 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(])Tj
-40.1602 -1.5591 Td
(Rearing)Tj
3.3733 0 Td
(\(DB\))Tj
ET
Q
q
1 j
1 J
0 w
5.5998 0 0 5.5998 235.3322 171.6661 cm
BT
/F13 1 Tf
1 TL
0 0 Td
()Tj
ET
Q
q
1 j
1 J
0 w
7.9999 0 0 7.9999 238.6204 168.7748 cm
BT
/F6 1 Tf
1 TL
0 0 Td
(^)Tj
5.1874 0 Td
(3.72)Tj
6.1937 0 Td
(0.00019)Tj
13.4151 0 Td
(1.6)Tj
9.5741 0 Td
([)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(])Tj
-40.1601 -1.5661 Td
(Grooming)Tj
4.4434 0 Td
(\(DB\))Tj
ET
Q
q
1 j
1 J
0 w
5.5998 0 0 5.5998 243.8929 159.137 cm
BT
/F13 1 Tf
1 TL
0 0 Td
()Tj
ET
Q
q
1 j
1 J
0 w
7.9999 0 0 7.9999 280.1196 156.2456 cm
BT
/F6 1 Tf
1 TL
0 0 Td
(3.1)Tj
6.1937 0 Td
(0.00079)Tj
22.9892 0 Td
([)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(])Tj
-40.1601 -1.559 Td
(Rearing)Tj
3.3733 0 Td
(\(OFT\))Tj
7.6039 0 Td
(8.24)Tj
6.1937 0 Td
(2.82E-10)Tj
13.4151 0 Td
(8.9)Tj
9.5741 0 Td
([)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(])Tj
ET
Q
q
1 j
1 J
0 w
5.5998 0 0 5.5998 36 127.8992 cm
BT
/F13 1 Tf
1 TL
0 0 Td
()Tj
ET
Q
q
1 j
1 J
0 w
7.9999 0 0 7.9999 41.1023 125.0078 cm
BT
/F6 1 Tf
1 TL
0 0 Td
(suggestive,)Tj
4.5354 0 Td
(trait)Tj
1.9063 0 Td
(x)Tj
0.7016 0 Td
(lineage)Tj
3.0544 0 Td
(covariates)Tj
-10.8355 -1.5023 Td
(^)Tj
0.7937 0 Td
(Rearing)Tj
3.3732 0 Td
(\(DB\))Tj
2.2323 0 Td
(QTL)Tj
2.1331 0 Td
(was)Tj
1.715 0 Td
(originally)Tj
4.0606 0 Td
(identified)Tj
4.0961 0 Td
(at)Tj
0.9638 0 Td
(62cM)Tj
-19.3678 -1.4953 Td
(From)Tj
2.459 0 Td
(the)Tj
1.4882 0 Td
(Rat)Tj
1.5945 0 Td
(Genome)Tj
3.678 0 Td
(Database)Tj
3.8693 0 Td
(version)Tj
3.1819 0 Td
(Rnor_6.0)Tj
0.83 0.64 0.02 0 k
/F9 1 Tf
-0.025 Tc
-16.2709 -2.2536 Td
(https://do)Tj
3.5575 0 Td
(i.org/10.1371/j)Tj
5.4425 0 Td
(ournal.pone)Tj
4.4079 0 Td
(.0194293.t001)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 349.1716 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Candidate)Tj
4.6913 0 Td
(quantitativ)Tj
4.4717 0 Td
(e)Tj
0.7654 0 Td
(trait)Tj
1.8567 0 Td
(genes)Tj
2.8984 0 Td
(for)Tj
1.3678 0 Td
(acute)Tj
2.622 0 Td
(stress)Tj
2.8489 0 Td
(responsive)Tj
4.748 0 Td
(ness)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01942)Tj
4.089 0 Td
(93)Tj
0 g
2.0906 0 Td
(March)Tj
2.9622 0 Td
(12,)Tj
1.5874 0 Td
(2018)Tj
34.3562 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(18)Tj
/F9 1 Tf
-0.025 Tc
-66.402 84.0904 Td
(conclusions)Tj
4.6559 0 Td
(contained)Tj
3.8268 0 Td
(herein)Tj
2.5158 0 Td
(are)Tj
1.3677 0 Td
(those)Tj
2.2677 0 Td
(of)Tj
0.9426 0 Td
(the)Tj
-15.5765 -1.3748 Td
(authors)Tj
3.0472 0 Td
(and)Tj
1.5874 0 Td
(should)Tj
2.7284 0 Td
(not)Tj
1.4244 0 Td
(be)Tj
1.1126 0 Td
(interpret)Tj
3.104 0 Td
(ed)Tj
1.1197 0 Td
(as)Tj
-14.1237 -1.3749 Td
(necessaril)Tj
3.6921 0 Td
(y)Tj
0.6449 0 Td
(representin)Tj
4.167 0 Td
(g)Tj
0.6944 0 Td
(the)Tj
1.3749 0 Td
(official)Tj
2.622 0 Td
(policies)Tj
3.0119 0 Td
(or)Tj
-16.2072 -1.3748 Td
(endorsemen)Tj
4.6417 0 Td
(ts,)Tj
1.1197 0 Td
(either)Tj
2.289 0 Td
(expressed)Tj
4.004 0 Td
(or)Tj
1.0063 0 Td
(implied,)Tj
3.1394 0 Td
(of)Tj
0.9496 0 Td
(Air)Tj
-17.1497 -1.3748 Td
(Force)Tj
2.2677 0 Td
(Researc)Tj
2.9976 0 Td
(h)Tj
0.6945 0 Td
(Laboratory)Tj
4.252 0 Td
(or)Tj
0.9993 0 Td
(the)Tj
1.3677 0 Td
(U.S.)Tj
-12.5788 -1.3748 Td
(Governmen)Tj
4.3299 0 Td
(t.)Tj
/F8 1 Tf
-4.3299 -2.126 Td
(Competing)Tj
4.3795 0 Td
(interests)Tj
3.3307 0 Td
(:)Tj
/F9 1 Tf
0.4819 0 Td
(The)Tj
1.5875 0 Td
(authors)Tj
3.0401 0 Td
(have)Tj
1.963 0 Td
(declared)Tj
-14.7827 -1.3748 Td
(that)Tj
1.6228 0 Td
(no)Tj
1.1693 0 Td
(competing)Tj
4.1599 0 Td
(interests)Tj
ET
endstream
endobj
47 0 obj
<>stream
endstream
endobj
48 0 obj
<>stream
endstream
endobj
49 0 obj
<>stream
endstream
endobj
50 0 obj
<>stream
endstream
endobj
51 0 obj
<>stream
endstream
endobj
52 0 obj
<>stream
endstream
endobj
53 0 obj
<>stream
endstream
endobj
54 0 obj
<>stream
endstream
endobj
55 0 obj
<>stream
endstream
endobj
56 0 obj
<>stream
endstream
endobj
57 0 obj
<>stream
endstream
endobj
58 0 obj
<>stream
endstream
endobj
59 0 obj
<>stream
endstream
endobj
60 0 obj
<>stream
endstream
endobj
61 0 obj
<>stream
BT
10.3394 73.7155 Td
(exist.)Tj
ET
Q
endstream
endobj
62 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 15 0 R/Annots 64 0 R/Contents 65 0 R/TrimBox[0 0 612 792]>>
endobj
64 0 obj
[66 0 R 67 0 R 68 0 R 69 0 R 70 0 R 71 0 R 72 0 R 73 0 R 74 0 R]
endobj
66 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref016)>>
endobj
67 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref019)>>
endobj
68 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.s001)>>
endobj
69 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref020)>>
endobj
70 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref021)>>
endobj
71 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref022)>>
endobj
72 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.s001)>>
endobj
73 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref023)>>
endobj
74 0 obj
<>/Border[0 0 0]/A 75 0 R>>
endobj
75 0 obj
<>
endobj
65 0 obj
[76 0 R 77 0 R 78 0 R 79 0 R 80 0 R 81 0 R 82 0 R 83 0 R 84 0 R 85 0 R 86 0 R 87 0 R]
endobj
76 0 obj
<>stream
q
0.83 0.64 0.02 0 k
269.5748 310.0535 m
278.8724 310.0535 l
h
f*
283.1811 310.0535 m
292.4787 310.0535 l
h
f*
458.5323 258.0661 m
491.6976 258.0661 l
h
f*
297.7512 232.0441 m
307.0488 232.0441 l
h
f*
394.1858 219.0614 m
403.4835 219.0614 l
h
f*
417.2031 193.0394 m
426.5008 193.0394 l
h
f*
432.1134 154.0346 m
465.4488 154.0346 l
h
f*
407.7354 81.9213 m
417.2031 81.9213 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F6 1 Tf
1 TL
-0.012 Tc
0 0 Td
(investigate)Tj
4.3709 0 Td
(the)Tj
1.4457 0 Td
(generalizability)Tj
6.1625 0 Td
(of)Tj
0.9978 0 Td
(the)Tj
1.4456 0 Td
(findings)Tj
3.4299 0 Td
(to)Tj
1.0092 0 Td
(a)Tj
0.6463 0 Td
(different)Tj
3.5829 0 Td
(acute)Tj
2.2847 0 Td
(stress)Tj
2.3471 0 Td
(phenotype,)Tj
4.5864 0 Td
(we)Tj
1.3096 0 Td
(measured)Tj
-33.6186 -1.3039 Td
(the)Tj
1.4456 0 Td
(behavioral)Tj
4.3086 0 Td
(response)Tj
3.6737 0 Td
(differences)Tj
4.4731 0 Td
(in)Tj
1.0204 0 Td
(the)Tj
1.4457 0 Td
(conditioning)Tj
5.3007 0 Td
(part)Tj
1.8085 0 Td
(of)Tj
0.9978 0 Td
(the)Tj
1.4457 0 Td
(contextual)Tj
4.3143 0 Td
(fear)Tj
1.7064 0 Td
(conditioning)Tj
-31.9405 -1.2982 Td
(\(CFC\))Tj
2.7155 0 Td
(paradigm)Tj
3.9912 0 Td
(between)Tj
3.4696 0 Td
(the)Tj
1.4456 0 Td
(two)Tj
1.6895 0 Td
(strains)Tj
2.8006 0 Td
(since)Tj
2.2053 0 Td
(the)Tj
1.4457 0 Td
(acute)Tj
2.2904 0 Td
(stress)Tj
2.347 0 Td
(of)Tj
0.9978 0 Td
(the)Tj
1.4457 0 Td
(electric)Tj
3.033 0 Td
(shock)Tj
2.4945 0 Td
(in)Tj
1.0205 0 Td
(the)Tj
1.4456 0 Td
(CFC)Tj
2.0523 0 Td
(is)Tj
-36.8898 -1.2983 Td
(similar)Tj
2.931 0 Td
(to)Tj
1.0091 0 Td
(that)Tj
1.7461 0 Td
(shock)Tj
2.4945 0 Td
(in)Tj
1.0205 0 Td
(the)Tj
1.4456 0 Td
(DB)Tj
1.5194 0 Td
(test.)Tj
1.7858 0 Td
(For)Tj
1.6044 0 Td
(proof-of-concept,)Tj
7.1376 0 Td
(we)Tj
1.3096 0 Td
(measured)Tj
4.0422 0 Td
(the)Tj
1.4456 0 Td
(transcript)Tj
4.0309 0 Td
(levels)Tj
2.33 0 Td
(of)Tj
-35.8523 -1.2983 Td
(these)Tj
2.211 0 Td
(candidate)Tj
4.0365 0 Td
(QTGs)Tj
2.6192 0 Td
(in)Tj
1.0204 0 Td
(the)Tj
1.4457 0 Td
(brain)Tj
2.3074 0 Td
(regions)Tj
3.1067 0 Td
(of)Tj
0.9978 0 Td
(a)Tj
0.6463 0 Td
(congenic)Tj
3.7644 0 Td
(strain)Tj
2.4548 0 Td
(generated)Tj
4.0592 0 Td
(by)Tj
1.1678 0 Td
(inserting)Tj
3.7021 0 Td
(the)Tj
/F10 1 Tf
-33.5393 -1.3039 Td
[()10()7()20()8()10()]TJ
/F6 1 Tf
3.3958 0 Td
(WKY)Tj
2.4662 0 Td
(donor)Tj
2.6418 0 Td
(region)Tj
2.7553 0 Td
(into)Tj
1.8085 0 Td
(the)Tj
1.4456 0 Td
(F344)Tj
2.143 0 Td
(recipient)Tj
3.6907 0 Td
(strain.)Tj
2.6702 0 Td
(DEGs)Tj
2.5682 0 Td
(with)Tj
1.9672 0 Td
(expression)Tj
4.3824 0 Td
(profiles)Tj
3.1407 0 Td
(in)Tj
1.0205 0 Td
(the)Tj
-36.0961 -1.2982 Td
(F344.WKY-)Tj
/F10 1 Tf
[()10()7()20()8()10()]TJ
/F6 1 Tf
8.124 0 Td
(congenic)Tj
3.7644 0 Td
(strain)Tj
2.4548 0 Td
(similar)Tj
2.931 0 Td
(to)Tj
1.0091 0 Td
(the)Tj
1.4457 0 Td
(WKY's,)Tj
3.2428 0 Td
(but)Tj
1.5307 0 Td
(different)Tj
3.5829 0 Td
(from)Tj
2.1714 0 Td
(the)Tj
1.4456 0 Td
(F344,)Tj
2.3641 0 Td
(suggest)Tj
-34.0665 -1.2983 Td
(that)Tj
1.7461 0 Td
(the)Tj
1.4456 0 Td
(sequence)Tj
3.7928 0 Td
(variations)Tj
4.0818 0 Td
(within)Tj
2.7666 0 Td
(these)Tj
2.2167 0 Td
(genes)Tj
2.3981 0 Td
(might)Tj
2.5568 0 Td
(contribute)Tj
4.3143 0 Td
(to)Tj
1.0092 0 Td
(the)Tj
/F10 1 Tf
1.4456 0 Td
[()10()7()20()8()10()]TJ
/F6 1 Tf
3.3959 0 Td
(QTL.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 587.2818 cm
BT
/F2 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Materials)Tj
4.5071 0 Td
(and)Tj
1.989 0 Td
(methods)Tj
/F11 1 Tf
-6.4961 -1.5023 Td
(Animal)Tj
3.3543 0 Td
(care)Tj
1.9418 0 Td
(and)Tj
1.7952 0 Td
(treatment)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 552.2456 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(All)Tj
1.3946 0 Td
(animal)Tj
2.9707 0 Td
(experiments)Tj
5.1363 0 Td
(were)Tj
2.126 0 Td
(performed)Tj
4.4674 0 Td
(according)Tj
4.1895 0 Td
(to)Tj
1.0262 0 Td
(procedures)Tj
4.6714 0 Td
(approved)Tj
3.9742 0 Td
(by)Tj
1.1792 0 Td
(the)Tj
1.4683 0 Td
(Institutional)Tj
-32.6038 -1.2982 Td
(Animal)Tj
3.2201 0 Td
(Care)Tj
2.109 0 Td
(and)Tj
1.7291 0 Td
(Use)Tj
1.7404 0 Td
(Committee)Tj
4.7452 0 Td
(of)Tj
1.0148 0 Td
(Northwestern)Tj
5.7713 0 Td
(University.)Tj
4.6261 0 Td
(Adult)Tj
2.5058 0 Td
(male)Tj
2.143 0 Td
(Fischer)Tj
3.1181 0 Td
(344)Tj
1.6497 0 Td
(\(F344\))Tj
-34.3726 -1.2983 Td
(and)Tj
1.7291 0 Td
(Wistar)Tj
2.9196 0 Td
(Kyoto)Tj
2.6589 0 Td
(\(WKY\))Tj
3.1691 0 Td
(rats)Tj
1.6838 0 Td
(\(approximately)Tj
6.3156 0 Td
(3)Tj
0.6973 0 Td
(months)Tj
3.2825 0 Td
(old\))Tj
1.8425 0 Td
(were)Tj
2.1203 0 Td
(obtained)Tj
3.736 0 Td
(from)Tj
2.2054 0 Td
(Envigo)Tj
-32.3601 -1.3039 Td
(\(Indianapolis,)Tj
5.7713 0 Td
(IN\))Tj
1.627 0 Td
(and)Tj
1.7235 0 Td
(Charles)Tj
3.2371 0 Td
(River)Tj
2.3471 0 Td
(Laboratories)Tj
5.2271 0 Td
(\(Wilmington,)Tj
5.7089 0 Td
(MA\),)Tj
2.3527 0 Td
(respectively.)Tj
5.1024 0 Td
(All)Tj
1.3889 0 Td
(animals)Tj
-34.486 -1.2983 Td
(were)Tj
2.1203 0 Td
(group-housed)Tj
5.8336 0 Td
(\(23)Tj
2.0296 0 Td
(per)Tj
1.5307 0 Td
(cage\))Tj
2.3074 0 Td
(in)Tj
1.0375 0 Td
(a)Tj
0.6519 0 Td
(temperature-controlled)Tj
9.5811 0 Td
(environment)Tj
5.4311 0 Td
(with)Tj
1.9956 0 Td
(12)Tj
1.1679 0 Td
(h)Tj
0.7483 0 Td
(light/)Tj
-34.435 -1.2982 Td
(dark)Tj
2.0466 0 Td
(cycles)Tj
2.5568 0 Td
(and)Tj
1.7291 0 Td
(allowed)Tj
3.2882 0 Td
(feed)Tj
1.8822 0 Td
(and)Tj
1.7291 0 Td
(water)Tj
/F10 1 Tf
2.4321 0 Td
[( )6(\n)12(\r)14()11(\r)14()7()12()]TJ
/F6 1 Tf
(.)Tj
-14.4679 -1.2983 Td
(The)Tj
1.7858 0 Td
(experimental)Tj
5.4595 0 Td
(design)Tj
2.8119 0 Td
(required)Tj
3.6397 0 Td
(three)Tj
2.262 0 Td
(animal)Tj
2.9707 0 Td
(cohorts.)Tj
3.4412 0 Td
(Specifically,)Tj
4.9096 0 Td
(one)Tj
1.7008 0 Td
(cohort)Tj
2.8573 0 Td
(of)Tj
1.0148 0 Td
(control)Tj
-34.0495 -1.3039 Td
(F344)Tj
2.1713 0 Td
(and)Tj
1.7348 0 Td
(WKY)Tj
2.4888 0 Td
(rats)Tj
1.6837 0 Td
(were)Tj
2.126 0 Td
(not)Tj
1.5704 0 Td
(subjected)Tj
3.9685 0 Td
(to)Tj
1.0261 0 Td
(any)Tj
1.6554 0 Td
(behavioral)Tj
4.3937 0 Td
(testing.)Tj
3.1068 0 Td
(RNA)Tj
2.262 0 Td
(was)Tj
1.7008 0 Td
(isolated)Tj
3.2825 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
-35.3761 -1.2983 Td
(three)Tj
2.262 0 Td
(brain)Tj
2.3471 0 Td
(regions)Tj
3.1634 0 Td
(and)Tj
1.7348 0 Td
(used)Tj
2.0579 0 Td
(for)Tj
1.3833 0 Td
(the)Tj
1.474 0 Td
(microarray)Tj
4.6715 0 Td
(experiments.)Tj
5.3631 0 Td
(The)Tj
1.7858 0 Td
(second)Tj
3.0104 0 Td
(cohort)Tj
2.8516 0 Td
(consisted)Tj
3.9402 0 Td
(of)Tj
-36.0451 -1.2982 Td
(F344)Tj
2.1713 0 Td
(and)Tj
1.7348 0 Td
(WKY)Tj
2.4888 0 Td
(rats)Tj
1.6837 0 Td
(that)Tj
1.7802 0 Td
(were)Tj
2.1203 0 Td
(subjected)Tj
3.9685 0 Td
(to)Tj
1.0261 0 Td
(CFC)Tj
2.0749 0 Td
(testing)Tj
2.88 0 Td
(and)Tj
1.7348 0 Td
(their)Tj
2.1033 0 Td
(RNA)Tj
2.262 0 Td
(was)Tj
1.7008 0 Td
(isolated)Tj
3.2882 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
-35.223 -1.304 Td
(three)Tj
2.262 0 Td
(brain)Tj
2.3471 0 Td
(regions.)Tj
3.3902 0 Td
(This)Tj
1.9899 0 Td
(RNA)Tj
2.262 0 Td
(was)Tj
1.6951 0 Td
(used)Tj
2.0636 0 Td
(for)Tj
1.3833 0 Td
(expression)Tj
4.4674 0 Td
(analyses)Tj
3.4809 0 Td
(by)Tj
1.1849 0 Td
(RT-PCR)Tj
3.6453 0 Td
(of)Tj
1.0148 0 Td
(candidate)Tj
4.1046 0 Td
(genes)Tj
-35.2911 -1.2982 Td
(together)Tj
3.5319 0 Td
(with)Tj
2.0012 0 Td
(RNA)Tj
2.2621 0 Td
(isolated)Tj
3.2825 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
1.4684 0 Td
(F344.WKY-)Tj
/F10 1 Tf
[()10()7()20()8()10()]TJ
/F6 1 Tf
8.2657 0 Td
(rats.)Tj
1.9106 0 Td
(The)Tj
1.7801 0 Td
(third)Tj
2.211 0 Td
(cohort)Tj
2.8573 0 Td
(of)Tj
1.0148 0 Td
(F344,)Tj
-32.7909 -1.2983 Td
(WKY,)Tj
2.7155 0 Td
(and)Tj
1.7291 0 Td
(F344.WKY-)Tj
/F10 1 Tf
[()10()7()20()8()10()]TJ
/F6 1 Tf
8.2658 0 Td
(rats)Tj
1.6838 0 Td
(were)Tj
2.1203 0 Td
(maintained)Tj
4.7905 0 Td
(independently)Tj
5.9754 0 Td
(and)Tj
1.7291 0 Td
(used)Tj
2.0636 0 Td
(for)Tj
1.3833 0 Td
(only)Tj
1.9786 0 Td
(behav-)Tj
-34.435 -1.3039 Td
(ioral)Tj
2.0466 0 Td
(testing)Tj
2.8799 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(OFT)Tj
2.1033 0 Td
(followed)Tj
3.651 0 Td
(by)Tj
1.1792 0 Td
(DB)Tj
1.5364 0 Td
(three)Tj
2.2677 0 Td
(weeks)Tj
2.6079 0 Td
(later.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 354.1606 cm
BT
/F11 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Construction)Tj
5.7685 0 Td
(of)Tj
1.0677 0 Td
(congenic)Tj
3.9071 0 Td
(F344.WKY-Stresp10)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 337.1527 cm
BT
/F6 1 Tf
1 TL
-0.015 Tc
0 0 Td
(The)Tj
1.7461 0 Td
(F344.WKY-)Tj
/F10 1 Tf
[()10()7()20()8()10()]TJ
/F6 1 Tf
8.0787 0 Td
(congenic)Tj
3.7417 0 Td
(strain)Tj
2.4321 0 Td
(was)Tj
1.6668 0 Td
(generated)Tj
4.0365 0 Td
(by)Tj
1.1565 0 Td
(repeated)Tj
3.5546 0 Td
(marker-directed)Tj
6.582 0 Td
(back-)Tj
-32.995 -1.3039 Td
(crossing)Tj
3.4355 0 Td
(of)Tj
0.9921 0 Td
(the)Tj
1.4344 0 Td
(F344-WKY)Tj
4.7111 0 Td
(F)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 310.9039 322.4692 cm
BT
/F6 1 Tf
1 TL
-0.02 Tc
0 0 Td
(1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 316.5732 324.1133 cm
BT
/F6 1 Tf
1 TL
-0.015 Tc
0 0 Td
(generation)Tj
4.3936 0 Td
(into)Tj
1.7972 0 Td
(the)Tj
1.44 0 Td
(F344)Tj
2.1316 0 Td
(parental)Tj
3.4016 0 Td
(strain.)Tj
2.6475 0 Td
(The)Tj
1.7518 0 Td
(markers)Tj
3.4186 0 Td
(used)Tj
2.0182 0 Td
(are)Tj
-34.656 -1.2982 Td
(described)Tj
3.9514 0 Td
(before)Tj
2.6759 0 Td
([)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.6895 0 Td
(19)Tj
0 g
(].)Tj
1.6951 0 Td
(The)Tj
1.7517 0 Td
(N)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 324.8503 309.4866 cm
BT
/F6 1 Tf
1 TL
-0.02 Tc
0 0 Td
(15)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 333.9779 311.1307 cm
BT
/F6 1 Tf
1 TL
-0.015 Tc
0 0 Td
(generation)Tj
4.3936 0 Td
(of)Tj
0.9978 0 Td
(F344.WKY-)Tj
/F10 1 Tf
[()10()7()20()8()10()]TJ
/F6 1 Tf
8.073 0 Td
(congenic)Tj
3.7417 0 Td
(male)Tj
2.0977 0 Td
(animals)Tj
-32.7002 -1.2982 Td
(\(approximately)Tj
6.1568 0 Td
(3)Tj
0.686 0 Td
(months)Tj
3.2145 0 Td
(old\))Tj
1.7971 0 Td
(were)Tj
2.0749 0 Td
(used)Tj
2.0182 0 Td
(for)Tj
1.3493 0 Td
(behavioral)Tj
4.2803 0 Td
(experimentation)Tj
6.667 0 Td
(together)Tj
3.4469 0 Td
(with)Tj
1.9503 0 Td
(the)Tj
1.4343 0 Td
(simul-)Tj
-35.0756 -1.3039 Td
(taneously)Tj
3.9231 0 Td
(maintained)Tj
4.6772 0 Td
(F344)Tj
2.1316 0 Td
(and)Tj
1.6951 0 Td
(WKY)Tj
2.4604 0 Td
(rats.)Tj
1.8538 0 Td
(The)Tj
1.7461 0 Td
(15)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 394.1858 289.5874 cm
BT
/F6 1 Tf
1 TL
-0.02 Tc
0 0 Td
(th)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 402.3496 285.1086 cm
BT
/F6 1 Tf
1 TL
-0.015 Tc
0 0 Td
(generation)Tj
4.3993 0 Td
(of)Tj
0.9921 0 Td
(the)Tj
1.4343 0 Td
(F344.WKY-)Tj
/F10 1 Tf
[()10()7()20()8()10()]TJ
/F6 1 Tf
8.0787 0 Td
(strain)Tj
-35.1379 -1.2982 Td
(was)Tj
1.6667 0 Td
(homozygous)Tj
5.1987 0 Td
(from)Tj
2.16 0 Td
(135,053)Tj
3.2258 0 Td
(to)Tj
0.9978 0 Td
(6,709,713)Tj
3.9061 0 Td
(bp)Tj
1.2189 0 Td
(and)Tj
1.7008 0 Td
(from)Tj
2.1599 0 Td
(19,464,437)Tj
4.3653 0 Td
(to)Tj
1.0035 0 Td
(the)Tj
1.4343 0 Td
(end)Tj
1.6894 0 Td
(of)Tj
0.9922 0 Td
(the)Tj
1.4343 0 Td
(QTL)Tj
2.0863 0 Td
(at)Tj
-35.24 -1.2983 Td
(28,931,796)Tj
4.3653 0 Td
(bp,)Tj
1.4343 0 Td
(and)Tj
1.7008 0 Td
(heterozygous)Tj
5.3631 0 Td
(from)Tj
2.1657 0 Td
(6,963,239)Tj
3.9004 0 Td
(to)Tj
1.0035 0 Td
(15,597,330)Tj
4.3652 0 Td
(bp)Tj
1.2246 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(S1)Tj
1.474 0 Td
(Table)Tj
0 g
(\).)Tj
2.931 0 Td
(Additionally,)Tj
5.3121 0 Td
(after)Tj
-35.24 -1.2982 Td
(15)Tj
1.1452 0 Td
(generations)Tj
4.7508 0 Td
(of)Tj
0.9921 0 Td
(backcross,)Tj
4.2179 0 Td
(congenic)Tj
3.7418 0 Td
(strains)Tj
2.7836 0 Td
(are)Tj
1.4173 0 Td
(known)Tj
2.9479 0 Td
(to)Tj
1.0034 0 Td
(have)Tj
2.0126 0 Td
(less)Tj
1.5704 0 Td
(then)Tj
1.9729 0 Td
(5%)Tj
1.4343 0 Td
(heterozygosity)Tj
-29.9902 -1.304 Td
(throughout)Tj
4.6658 0 Td
(the)Tj
1.44 0 Td
(genome)Tj
3.3392 0 Td
([)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
(].)Tj
2.0239 0 Td
(This)Tj
1.9389 0 Td
(strain)Tj
2.4377 0 Td
(was)Tj
1.6668 0 Td
(characterized)Tj
5.414 0 Td
(using)Tj
2.3358 0 Td
(the)Tj
1.4343 0 Td
(10K)Tj
1.8142 0 Td
(Affymetrix)Tj
4.4673 0 Td
(Targeted)Tj
-32.9779 -1.2982 Td
(Genotyping)Tj
4.8529 0 Td
(Array)Tj
2.4774 0 Td
(\(Affymetrix,)Tj
5.006 0 Td
(Santa)Tj
2.3527 0 Td
(Clara,)Tj
2.5285 0 Td
(CA\))Tj
1.8709 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(].)Tj
2.0181 0 Td
(Using)Tj
2.5398 0 Td
(this)Tj
1.6271 0 Td
(array,)Tj
2.4378 0 Td
(4)Tj
/F14 1 Tf
0.6803 0 Td
[()]TJ
/F6 1 Tf
(g)Tj
1.1679 0 Td
(of)Tj
0.9921 0 Td
(rat)Tj
1.2869 0 Td
(DNA)Tj
2.3414 0 Td
(was)Tj
1.6611 0 Td
(gen-)Tj
-35.8409 -1.2983 Td
(otyped)Tj
2.88 0 Td
(with)Tj
1.9559 0 Td
(the)Tj
1.4343 0 Td
(GeneChip)Tj
4.2179 0 Td
(Scanner)Tj
3.3675 0 Td
(2000)Tj
2.0807 0 Td
(Targeted)Tj
3.685 0 Td
(Genotyping)Tj
4.8584 0 Td
(System)Tj
2.9877 0 Td
(\(GTGS\))Tj
3.3506 0 Td
(following)Tj
3.8777 0 Td
(the)Tj
-34.6957 -1.3039 Td
(manufacturer's)Tj
6.1058 0 Td
(protocol)Tj
3.5092 0 Td
(\(Affymetrix,)Tj
5.0173 0 Td
(Santa)Tj
2.3528 0 Td
(Clara,)Tj
2.5285 0 Td
(CA\))Tj
1.8764 0 Td
([)Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
(].)Tj
2.0182 0 Td
(The)Tj
1.7518 0 Td
(genotyping)Tj
4.6035 0 Td
(data)Tj
1.8708 0 Td
(was)Tj
1.6668 0 Td
(analyzed)Tj
-33.3011 -1.2983 Td
(using)Tj
2.3357 0 Td
(the)Tj
1.4343 0 Td
(corresponding)Tj
5.9187 0 Td
(software)Tj
3.498 0 Td
(for)Tj
1.3492 0 Td
(GTGS)Tj
2.6986 0 Td
(\(Affymetrix,)Tj
5.0115 0 Td
(Santa)Tj
2.3528 0 Td
(Clara,)Tj
2.5285 0 Td
(CA\).)Tj
2.0919 0 Td
(The)Tj
1.7462 0 Td
(10K)Tj
1.8141 0 Td
(Affymetrix)Tj
-32.7795 -1.2982 Td
(Targeted)Tj
3.685 0 Td
(Genotyping)Tj
4.8585 0 Td
(Array)Tj
2.4775 0 Td
(used)Tj
2.0183 0 Td
(the)Tj
1.4343 0 Td
(rat)Tj
1.2926 0 Td
(genome)Tj
3.3392 0 Td
(version)Tj
3.0783 0 Td
(RGSC_3.4.)Tj
4.4957 0 Td
(The)Tj
1.7461 0 Td
(QTL)Tj
2.0863 0 Td
(locations)Tj
3.7134 0 Td
(were)Tj
-34.2252 -1.304 Td
(translated)Tj
4.0535 0 Td
(to)Tj
1.0035 0 Td
(the)Tj
1.4343 0 Td
(latest)Tj
2.2167 0 Td
(version)Tj
3.0727 0 Td
(or)Tj
1.0771 0 Td
(the)Tj
1.44 0 Td
(rat)Tj
1.287 0 Td
(genome,)Tj
3.5603 0 Td
(Rnor_6.0.)Tj
0.83 0.64 0.02 0 k
4.0647 0 Td
(S1)Tj
1.1509 0 Td
(Table)Tj
0 g
2.3867 0 Td
(lists)Tj
1.6951 0 Td
(the)Tj
1.44 0 Td
(QTL)Tj
2.0806 0 Td
(locations)Tj
-31.9631 -1.2982 Td
(from)Tj
2.16 0 Td
(both)Tj
2.0182 0 Td
(versions)Tj
3.4242 0 Td
(of)Tj
0.9922 0 Td
(the)Tj
1.44 0 Td
(rat)Tj
1.2869 0 Td
(genome.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 112.9889 cm
BT
/F11 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Behavioral)Tj
4.7149 0 Td
(tests)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 211.9748 95.9811 cm
BT
/F11 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Contextual)Tj
4.8528 0 Td
(fear)Tj
1.8539 0 Td
(conditioning.)Tj
/F6 1 Tf
6.4176 0 Td
(The)Tj
1.7858 0 Td
(fear)Tj
1.7348 0 Td
(conditioning)Tj
5.3971 0 Td
(phase)Tj
2.4831 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(contextual)Tj
4.3937 0 Td
(fear)Tj
1.7347 0 Td
(condi-)Tj
-34.3329 -1.2982 Td
(tioning)Tj
3.118 0 Td
(test)Tj
1.6044 0 Td
(was)Tj
1.7008 0 Td
(carried)Tj
3.0387 0 Td
(out)Tj
1.5477 0 Td
(as)Tj
1.0148 0 Td
(described)Tj
4.0535 0 Td
(previously)Tj
4.354 0 Td
([)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(].)Tj
2.075 0 Td
(Briefly,)Tj
3.0784 0 Td
(male)Tj
2.143 0 Td
(rats)Tj
1.6837 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(F344)Tj
2.177 0 Td
(and)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 349.1716 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Candidate)Tj
4.6913 0 Td
(quantitativ)Tj
4.4717 0 Td
(e)Tj
0.7654 0 Td
(trait)Tj
1.8567 0 Td
(genes)Tj
2.8984 0 Td
(for)Tj
1.3678 0 Td
(acute)Tj
2.622 0 Td
(stress)Tj
2.8489 0 Td
(responsive)Tj
4.748 0 Td
(ness)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01942)Tj
4.089 0 Td
(93)Tj
0 g
2.0906 0 Td
(March)Tj
2.9622 0 Td
(12,)Tj
1.5874 0 Td
(2018)Tj
34.3562 0 Td
(3)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
77 0 obj
<>stream
endstream
endobj
78 0 obj
<>stream
endstream
endobj
79 0 obj
<>stream
endstream
endobj
80 0 obj
<>stream
endstream
endobj
81 0 obj
<>stream
endstream
endobj
82 0 obj
<>stream
endstream
endobj
83 0 obj
<>stream
endstream
endobj
84 0 obj
<>stream
endstream
endobj
85 0 obj
<>stream
endstream
endobj
86 0 obj
<>stream
endstream
endobj
87 0 obj
<>stream
BT
66.402 0 Td
(18)Tj
ET
Q
endstream
endobj
88 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 15 0 R/Annots 89 0 R/Contents 90 0 R/TrimBox[0 0 612 792]>>
endobj
89 0 obj
[91 0 R 92 0 R 93 0 R 94 0 R 95 0 R 96 0 R 97 0 R]
endobj
91 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref023)>>
endobj
92 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref015)>>
endobj
93 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref016)>>
endobj
94 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref019)>>
endobj
95 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref024)>>
endobj
96 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref025)>>
endobj
97 0 obj
<>/Border[0 0 0]/A 98 0 R>>
endobj
98 0 obj
<>
endobj
90 0 obj
[99 0 R 100 0 R 101 0 R 102 0 R 103 0 R 104 0 R 105 0 R 106 0 R]
endobj
99 0 obj
<>stream
q
0.83 0.64 0.02 0 k
267.0803 563.3008 m
276.6047 563.3008 l
h
f*
457.0016 459.326 m
466.526 459.326 l
h
f*
470.948 459.326 m
480.4724 459.326 l
h
f*
484.9512 459.326 m
494.4189 459.326 l
h
f*
497.8205 211.9181 m
507.2315 211.9181 l
h
f*
203.3575 172.9134 m
212.7118 172.9134 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(WKY)Tj
2.4888 0 Td
(strains)Tj
2.8629 0 Td
(were)Tj
2.1203 0 Td
(individually)Tj
4.989 0 Td
(placed)Tj
2.7949 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(CFC)Tj
2.075 0 Td
(chamber)Tj
3.7247 0 Td
(\(Technical)Tj
4.4673 0 Td
(&)Tj
0.9241 0 Td
(Scientific)Tj
3.9005 0 Td
(Equipment,)Tj
-32.8533 -1.3039 Td
(Bad)Tj
1.7574 0 Td
(Homburg,)Tj
4.4164 0 Td
(Germany\))Tj
4.3256 0 Td
(and)Tj
1.7291 0 Td
(exposed)Tj
3.4469 0 Td
(to)Tj
1.0262 0 Td
(3)Tj
0.6916 0 Td
(minutes)Tj
3.4696 0 Td
(of)Tj
1.0148 0 Td
(habituation)Tj
4.8359 0 Td
(followed)Tj
3.6453 0 Td
(by)Tj
1.1849 0 Td
(3)Tj
0.6973 0 Td
(footshocks)Tj
-32.241 -1.2982 Td
(\(0.8)Tj
1.7347 0 Td
(mA,)Tj
1.9503 0 Td
(1)Tj
0.6973 0 Td
(sec)Tj
1.4286 0 Td
(duration,)Tj
3.9232 0 Td
(spaced)Tj
2.9083 0 Td
(1)Tj
0.6973 0 Td
(minute)Tj
3.1011 0 Td
(apart\).)Tj
2.8403 0 Td
(The)Tj
1.7858 0 Td
(behavior)Tj
3.7134 0 Td
(observed)Tj
3.7927 0 Td
(after)Tj
2.0353 0 Td
(the)Tj
1.4683 0 Td
(footshocks)Tj
-32.0766 -1.2983 Td
(was)Tj
1.6951 0 Td
(analyzed)Tj
3.702 0 Td
(by)Tj
1.1849 0 Td
(the)Tj
1.4683 0 Td
(TSE)Tj
1.8765 0 Td
(Videomot)Tj
4.2633 0 Td
(software)Tj
3.5886 0 Td
(version)Tj
3.1521 0 Td
(5.75)Tj
1.8709 0 Td
(\(Bad)Tj
2.1033 0 Td
(Homburg,)Tj
4.4107 0 Td
(Germany\),)Tj
4.5524 0 Td
(which)Tj
-33.8681 -1.2983 Td
(measures)Tj
3.9401 0 Td
(the)Tj
1.4683 0 Td
(animals')Tj
3.549 0 Td
(movement,)Tj
4.7395 0 Td
(including)Tj
4.0422 0 Td
(distance)Tj
3.5036 0 Td
(travelled,)Tj
3.8721 0 Td
(number)Tj
3.4128 0 Td
(of)Tj
1.0148 0 Td
(times)Tj
2.3925 0 Td
(rearing,)Tj
3.3278 0 Td
(and)Tj
-35.2627 -1.3039 Td
(freezing)Tj
3.4185 0 Td
(duration.)Tj
3.9288 0 Td
(The)Tj
1.7802 0 Td
(rats)Tj
1.6837 0 Td
(were)Tj
2.126 0 Td
(sacrificed)Tj
4.0025 0 Td
(by)Tj
1.1849 0 Td
(quick)Tj
2.4434 0 Td
(decapitation)Tj
5.1647 0 Td
(24)Tj
1.1679 0 Td
(hours)Tj
2.5171 0 Td
(later.)Tj
2.2167 0 Td
(Whole)Tj
2.8913 0 Td
(brains)Tj
-34.5257 -1.2982 Td
(were)Tj
2.1203 0 Td
(collected)Tj
3.7417 0 Td
(in)Tj
1.0318 0 Td
(RNAlater)Tj
4.0365 0 Td
(\(Ambion,)Tj
4.1215 0 Td
(Austin,)Tj
3.1295 0 Td
(TX\))Tj
1.8255 0 Td
(and)Tj
1.7291 0 Td
(frozen)Tj
2.7722 0 Td
(at)Tj
0.9525 0 Td
(-80C)Tj
2.5795 0 Td
(until)Tj
2.1033 0 Td
(dissection)Tj
4.2066 0 Td
(of)Tj
1.0148 0 Td
(brain)Tj
-35.3648 -1.2983 Td
(regions.)Tj
3.3902 0 Td
(Total)Tj
2.313 0 Td
(RNA)Tj
2.2621 0 Td
(was)Tj
1.7007 0 Td
(isolated)Tj
3.2825 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.474 0 Td
(hippocampus,)Tj
5.862 0 Td
(amygdala,)Tj
4.2633 0 Td
(and)Tj
1.7291 0 Td
(frontal)Tj
2.914 0 Td
(cortex,)Tj
2.931 0 Td
(and)Tj
1.7291 0 Td
(was)Tj
-36.0564 -1.3039 Td
(used)Tj
2.0579 0 Td
(for)Tj
1.389 0 Td
(the)Tj
1.4683 0 Td
(expression)Tj
4.4617 0 Td
(analyses)Tj
3.4866 0 Td
(of)Tj
1.0148 0 Td
(candidate)Tj
4.1045 0 Td
(genes.)Tj
2.6646 0 Td
(All)Tj
1.4003 0 Td
(rats)Tj
1.6838 0 Td
(were)Tj
2.1203 0 Td
(approximately)Tj
5.9754 0 Td
(3)Tj
0.6973 0 Td
(months)Tj
3.2825 0 Td
(old)Tj
-35.807 -1.2983 Td
(at)Tj
0.9524 0 Td
(the)Tj
1.4683 0 Td
(time)Tj
2.0296 0 Td
(of)Tj
1.0148 0 Td
(sacrifice)Tj
3.4753 0 Td
(and)Tj
1.7348 0 Td
(tissue)Tj
2.4547 0 Td
(sample)Tj
3.0218 0 Td
(collection.)Tj
/F11 1 Tf
-14.9555 -1.2983 Td
(Open)Tj
2.5285 0 Td
(field)Tj
2.0919 0 Td
(test.)Tj
/F6 1 Tf
2.5398 0 Td
(The)Tj
1.7802 0 Td
(open)Tj
2.211 0 Td
(field)Tj
1.9672 0 Td
(test)Tj
1.5988 0 Td
(was)Tj
1.6951 0 Td
(carried)Tj
3.033 0 Td
(out)Tj
1.5477 0 Td
(on)Tj
1.2699 0 Td
(another)Tj
3.3279 0 Td
(cohort)Tj
2.8516 0 Td
(of)Tj
1.0091 0 Td
(animals,)Tj
3.549 0 Td
(as)Tj
1.0148 0 Td
(previ-)Tj
-35.2117 -1.3039 Td
(ously)Tj
2.313 0 Td
(described)Tj
4.0535 0 Td
([)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(].)Tj
2.075 0 Td
(Briefly,)Tj
3.0784 0 Td
(male)Tj
2.143 0 Td
(rats)Tj
1.6837 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(F344,)Tj
2.3981 0 Td
(WKY,)Tj
2.7156 0 Td
(and)Tj
1.7291 0 Td
(F344.WKY-)Tj
/F10 1 Tf
[()10()7()20()8()10()]TJ
/F6 1 Tf
8.2658 0 Td
(strains)Tj
-32.944 -1.2982 Td
(were)Tj
2.1203 0 Td
(placed)Tj
2.7949 0 Td
(in)Tj
1.0318 0 Td
(a)Tj
0.6576 0 Td
(circular)Tj
3.2825 0 Td
(arena)Tj
2.4321 0 Td
(\(82)Tj
1.5137 0 Td
(cm)Tj
1.4627 0 Td
(in)Tj
1.0318 0 Td
(diameter\))Tj
4.1272 0 Td
(with)Tj
1.9956 0 Td
(a)Tj
0.6576 0 Td
(30)Tj
1.1679 0 Td
(cm)Tj
1.4627 0 Td
(high)Tj
2.0069 0 Td
(wall,)Tj
2.0579 0 Td
(lit)Tj
1.0318 0 Td
(to)Tj
1.0262 0 Td
(a)Tj
0.6519 0 Td
(brightness)Tj
-32.5131 -1.2983 Td
(of)Tj
1.0147 0 Td
(approximately)Tj
5.9754 0 Td
(60)Tj
1.1679 0 Td
(lux)Tj
1.4627 0 Td
(by)Tj
1.1792 0 Td
(indirect)Tj
3.3505 0 Td
(overhead)Tj
3.8891 0 Td
(lighting.)Tj
3.532 0 Td
(The)Tj
1.7801 0 Td
(arena)Tj
2.4321 0 Td
(contained)Tj
4.1953 0 Td
(an)Tj
1.2019 0 Td
(inner)Tj
2.381 0 Td
(concen-)Tj
-33.5619 -1.3039 Td
(tric)Tj
1.5817 0 Td
(circle)Tj
2.3697 0 Td
(\(50)Tj
1.5137 0 Td
(cm)Tj
1.4627 0 Td
(in)Tj
1.0375 0 Td
(diameter\))Tj
4.1215 0 Td
(designated)Tj
4.5014 0 Td
(as)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(inner)Tj
2.3811 0 Td
(zone.)Tj
2.3357 0 Td
(The)Tj
1.7802 0 Td
(rats)Tj
1.6838 0 Td
(were)Tj
2.1259 0 Td
(placed)Tj
2.7893 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(center)Tj
-34.6731 -1.2983 Td
(of)Tj
1.0147 0 Td
(the)Tj
1.4684 0 Td
(arena)Tj
2.4321 0 Td
(and)Tj
1.7291 0 Td
(allowed)Tj
3.2882 0 Td
(to)Tj
1.0261 0 Td
(move)Tj
2.4265 0 Td
(freely)Tj
2.4264 0 Td
(for)Tj
1.3833 0 Td
(10)Tj
1.1735 0 Td
(min)Tj
1.8539 0 Td
(while)Tj
2.3697 0 Td
(the)Tj
1.4684 0 Td
(activity)Tj
3.1181 0 Td
(was)Tj
1.7007 0 Td
(recorded)Tj
3.7928 0 Td
(and)Tj
1.7348 0 Td
(tracked)Tj
-34.4067 -1.2982 Td
(by)Tj
1.1792 0 Td
(the)Tj
1.4683 0 Td
(TSE)Tj
1.8765 0 Td
(Videomot)Tj
4.269 0 Td
(software)Tj
3.5829 0 Td
(2)Tj
0.6974 0 Td
(version)Tj
3.1521 0 Td
(5.75)Tj
1.8708 0 Td
(\(Bad)Tj
2.1033 0 Td
(Homburg,)Tj
4.4107 0 Td
(Germany\),)Tj
4.5524 0 Td
(which)Tj
2.6532 0 Td
(measured)Tj
4.1046 0 Td
(the)Tj
-35.9204 -1.2983 Td
(number)Tj
3.4185 0 Td
(of)Tj
1.0148 0 Td
(times)Tj
2.3868 0 Td
(rearing)Tj
3.101 0 Td
(by)Tj
1.1849 0 Td
(the)Tj
1.4684 0 Td
(animal.)Tj
/F11 1 Tf
-11.3782 -1.3039 Td
(Defensive)Tj
4.3029 0 Td
(burying.)Tj
/F6 1 Tf
4.3257 0 Td
(Three)Tj
2.5795 0 Td
(weeks)Tj
2.6078 0 Td
(after)Tj
2.0353 0 Td
(the)Tj
1.4683 0 Td
(OFT,)Tj
2.3301 0 Td
(the)Tj
1.4683 0 Td
(defensive)Tj
3.9402 0 Td
(burying)Tj
3.3618 0 Td
(test)Tj
1.5988 0 Td
(was)Tj
1.7007 0 Td
(carried)Tj
3.0388 0 Td
(out)Tj
-35.9544 -1.2983 Td
(on)Tj
1.2755 0 Td
(the)Tj
1.4684 0 Td
(same)Tj
2.2563 0 Td
(animals)Tj
3.3336 0 Td
(as)Tj
1.0148 0 Td
(previously)Tj
4.3483 0 Td
(described)Tj
4.0535 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(QTL)Tj
2.1146 0 Td
(studies)Tj
2.9877 0 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(16)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.4003 0 Td
(19)Tj
0 g
(].)Tj
1.7348 0 Td
(Briefly,)Tj
3.0728 0 Td
(male)Tj
2.1486 0 Td
(rats)Tj
-35.4498 -1.2983 Td
(of)Tj
1.0147 0 Td
(the)Tj
1.4684 0 Td
(three)Tj
2.262 0 Td
(strains)Tj
2.863 0 Td
(were)Tj
2.126 0 Td
(habituated)Tj
4.4617 0 Td
(to)Tj
1.0261 0 Td
(a)Tj
0.652 0 Td
(Plexiglas)Tj
3.6907 0 Td
(chamber)Tj
3.7247 0 Td
(\(40)Tj
1.5136 0 Td
(cm)Tj
1.4627 0 Td
(square,)Tj
3.0671 0 Td
(60)Tj
1.1735 0 Td
(cm)Tj
1.4627 0 Td
(high\))Tj
2.3471 0 Td
(with)Tj
-34.316 -1.3039 Td
(bedding)Tj
3.4865 0 Td
(\(wood)Tj
2.7836 0 Td
(shaving\))Tj
3.617 0 Td
(\(7)Tj
1.0375 0 Td
(cm)Tj
1.4627 0 Td
(deep,)Tj
2.3357 0 Td
(1)Tj
0.6973 0 Td
(cm)Tj
1.4627 0 Td
(below)Tj
2.5908 0 Td
(the)Tj
1.4684 0 Td
(hole)Tj
1.9219 0 Td
(for)Tj
1.3889 0 Td
(the)Tj
1.4684 0 Td
(prod\))Tj
2.4831 0 Td
(for)Tj
1.389 0 Td
(15)Tj
1.1678 0 Td
(min)Tj
1.8595 0 Td
(each)Tj
2.024 0 Td
(day,)Tj
-34.6448 -1.2982 Td
(for)Tj
1.3832 0 Td
(three)Tj
2.2678 0 Td
(consecutive)Tj
4.8755 0 Td
(days,)Tj
2.2224 0 Td
(between)Tj
3.5319 0 Td
(10:00)Tj
2.3471 0 Td
(AM)Tj
1.7971 0 Td
(and)Tj
1.7292 0 Td
(2:00)Tj
1.8708 0 Td
(PM.)Tj
1.8992 0 Td
(On)Tj
1.5137 0 Td
(the)Tj
1.4683 0 Td
(fourth)Tj
2.744 0 Td
(day,)Tj
1.8595 0 Td
(a)Tj
0.6519 0 Td
(continuously)Tj
-32.1616 -1.2983 Td
(electrified)Tj
4.1782 0 Td
(prod)Tj
2.143 0 Td
(was)Tj
1.7007 0 Td
(introduced)Tj
4.6432 0 Td
(into)Tj
1.8368 0 Td
(the)Tj
1.474 0 Td
(chamber,)Tj
3.9515 0 Td
(which)Tj
2.6475 0 Td
(delivered)Tj
3.8891 0 Td
(a)Tj
0.652 0 Td
(4.5)Tj
1.3946 0 Td
(mA)Tj
1.7235 0 Td
(shock)Tj
2.5398 0 Td
(when)Tj
2.4038 0 Td
(the)Tj
-35.1777 -1.3039 Td
(rat)Tj
1.3209 0 Td
(touched)Tj
3.4582 0 Td
(it.)Tj
1.0092 0 Td
(The)Tj
1.7801 0 Td
(shock)Tj
2.5398 0 Td
(was)Tj
1.6951 0 Td
(generated)Tj
4.1386 0 Td
(from)Tj
2.2053 0 Td
(a)Tj
0.652 0 Td
(shock)Tj
2.5398 0 Td
(generator)Tj
4.0592 0 Td
(\(Lafayette)Tj
4.1556 0 Td
(Instruments,)Tj
5.3404 0 Td
(San)Tj
-34.8942 -1.2983 Td
(Diego,)Tj
2.8402 0 Td
(CA\))Tj
1.9106 0 Td
(set)Tj
1.3039 0 Td
(at)Tj
0.9524 0 Td
(4.5)Tj
1.3947 0 Td
(mA.)Tj
1.9502 0 Td
(The)Tj
1.7802 0 Td
(rats)Tj
1.6837 0 Td
(typically)Tj
3.566 0 Td
(explored)Tj
3.702 0 Td
(the)Tj
1.4683 0 Td
(novel)Tj
2.3981 0 Td
(prod)Tj
2.143 0 Td
(and)Tj
1.7348 0 Td
(received)Tj
3.532 0 Td
(a)Tj
0.6519 0 Td
(shock,)Tj
-33.012 -1.2983 Td
(which)Tj
2.6475 0 Td
(started)Tj
2.9367 0 Td
(the)Tj
1.4683 0 Td
(15-minute)Tj
4.405 0 Td
(video-taped)Tj
4.9549 0 Td
(test.)Tj
1.8255 0 Td
(Once)Tj
2.3641 0 Td
(shocked,)Tj
3.7077 0 Td
(animals)Tj
3.3335 0 Td
(typically)Tj
3.5603 0 Td
(retreated)Tj
3.7928 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
-36.0224 -1.2982 Td
(back)Tj
2.0806 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(cage,)Tj
2.194 0 Td
(and)Tj
1.7291 0 Td
(either)Tj
2.5285 0 Td
(remained)Tj
4.0365 0 Td
(there)Tj
2.2677 0 Td
(\(classic)Tj
3.0728 0 Td
(WKY)Tj
2.4944 0 Td
(behavior\))Tj
4.0479 0 Td
(or)Tj
1.0998 0 Td
(began)Tj
2.5965 0 Td
(spraying)Tj
3.6397 0 Td
(bedding)Tj
-34.2706 -1.3039 Td
(toward)Tj
3.0387 0 Td
(the)Tj
1.474 0 Td
(prod)Tj
2.143 0 Td
(in)Tj
1.0318 0 Td
(an)Tj
1.2075 0 Td
(effort)Tj
2.3924 0 Td
(to)Tj
1.0262 0 Td
(cover)Tj
2.3981 0 Td
(it.)Tj
1.0148 0 Td
(Behaviors)Tj
4.1442 0 Td
(scored)Tj
2.8346 0 Td
(by)Tj
1.1792 0 Td
(an)Tj
1.2019 0 Td
(observer)Tj
3.6397 0 Td
(blind)Tj
2.3187 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(genotype)Tj
-33.5393 -1.2983 Td
(of)Tj
1.0147 0 Td
(the)Tj
1.4684 0 Td
(animal)Tj
2.9707 0 Td
(include)Tj
3.1804 0 Td
(the)Tj
1.474 0 Td
(latency)Tj
3.0501 0 Td
(to)Tj
1.0205 0 Td
(begin)Tj
2.4321 0 Td
(burying,)Tj
3.5886 0 Td
(the)Tj
1.4683 0 Td
(total)Tj
2.007 0 Td
(time)Tj
2.0239 0 Td
(spent)Tj
2.3697 0 Td
(burying)Tj
3.3619 0 Td
(\(duration)Tj
4.0422 0 Td
(of)Tj
-35.4725 -1.2983 Td
(burying\),)Tj
3.9231 0 Td
(the)Tj
1.474 0 Td
(duration)Tj
3.702 0 Td
(of)Tj
1.0148 0 Td
(grooming,)Tj
4.3937 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.474 0 Td
(number)Tj
3.4129 0 Td
(of)Tj
1.0148 0 Td
(times)Tj
2.3924 0 Td
(rearing)Tj
3.1011 0 Td
(by)Tj
1.1792 0 Td
(the)Tj
1.474 0 Td
(animal.)Tj
3.1918 0 Td
(The)Tj
1.7802 0 Td
(rats)Tj
-35.2571 -1.3039 Td
(were)Tj
2.1203 0 Td
(sacrificed)Tj
4.0081 0 Td
(by)Tj
1.1792 0 Td
(quick)Tj
2.4435 0 Td
(decapitation)Tj
5.1647 0 Td
(two)Tj
1.7121 0 Td
(weeks)Tj
2.6078 0 Td
(after)Tj
2.0353 0 Td
(the)Tj
1.4683 0 Td
(test.)Tj
1.8255 0 Td
(Whole)Tj
2.8914 0 Td
(brains)Tj
2.7099 0 Td
(were)Tj
2.1203 0 Td
(collected)Tj
3.7417 0 Td
(in)Tj
-36.0281 -1.2982 Td
(RNAlater)Tj
4.0365 0 Td
(\(Ambion,)Tj
4.1158 0 Td
(Austin,)Tj
3.1351 0 Td
(TX\))Tj
1.8199 0 Td
(and)Tj
1.7348 0 Td
(frozen)Tj
2.7666 0 Td
(at)Tj
0.9524 0 Td
(-80C)Tj
2.5795 0 Td
(until)Tj
2.109 0 Td
(dissection.)Tj
4.4277 0 Td
(All)Tj
1.4003 0 Td
(rats)Tj
1.6837 0 Td
(were)Tj
2.1203 0 Td
(approxi-)Tj
-32.8816 -1.2983 Td
(mately)Tj
2.8969 0 Td
(3)Tj
0.6917 0 Td
(months)Tj
3.2825 0 Td
(old)Tj
1.5023 0 Td
(at)Tj
0.9525 0 Td
(the)Tj
1.4683 0 Td
(time)Tj
2.0296 0 Td
(of)Tj
1.0148 0 Td
(sacrifice)Tj
3.4809 0 Td
(and)Tj
1.7291 0 Td
(tissue)Tj
2.4605 0 Td
(sample)Tj
3.0217 0 Td
(collection.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 242.9858 cm
BT
/F11 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Brain)Tj
2.5653 0 Td
(dissection)Tj
4.3796 0 Td
(and)Tj
1.7906 0 Td
(RNA)Tj
2.3149 0 Td
(isolation)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 225.9779 cm
BT
/F6 1 Tf
1 TL
-0.01 Tc
0 0 Td
(Brain)Tj
2.3924 0 Td
(regions)Tj
3.1181 0 Td
(\(hippocampus,)Tj
6.1284 0 Td
(amygdala,)Tj
4.2066 0 Td
(and)Tj
1.7121 0 Td
(frontal)Tj
2.8743 0 Td
(cortex\))Tj
3.0048 0 Td
(were)Tj
2.0976 0 Td
(dissected)Tj
3.7814 0 Td
(from)Tj
2.1826 0 Td
(adult)Tj
2.2224 0 Td
(male)Tj
-33.7207 -1.2982 Td
(F344,)Tj
2.3697 0 Td
(WKY)Tj
2.4718 0 Td
(and)Tj
1.7121 0 Td
(F344.WKY-)Tj
/F10 1 Tf
[()10()7()20()8()10()]TJ
/F6 1 Tf
8.1637 0 Td
(congenic)Tj
3.7758 0 Td
(rats)Tj
1.6611 0 Td
(as)Tj
1.0034 0 Td
(described)Tj
4.0025 0 Td
(previously)Tj
4.286 0 Td
([)Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
(])Tj
1.8311 0 Td
(and)Tj
1.7121 0 Td
(stored)Tj
2.6703 0 Td
(in)Tj
-35.6596 -1.2983 Td
(RNAlater)Tj
3.9854 0 Td
(\(Ambion,)Tj
4.0706 0 Td
(Austin,)Tj
3.0897 0 Td
(TX\))Tj
1.8085 0 Td
(at)Tj
0.9411 0 Td
(-80C.)Tj
2.7666 0 Td
(Briefly,)Tj
3.0274 0 Td
(a)Tj
0.6463 0 Td
(rat)Tj
1.3096 0 Td
(brain)Tj
2.313 0 Td
(matrix)Tj
2.8403 0 Td
(was)Tj
1.6838 0 Td
(used)Tj
2.0353 0 Td
(to)Tj
1.0147 0 Td
(prepare)Tj
3.2372 0 Td
(pri-)Tj
-34.7695 -1.3039 Td
(mary)Tj
2.2733 0 Td
(sections)Tj
3.3619 0 Td
(from)Tj
2.1827 0 Td
(which)Tj
2.6191 0 Td
(the)Tj
1.4514 0 Td
(brain)Tj
2.3187 0 Td
(regions)Tj
3.1238 0 Td
(were)Tj
2.0976 0 Td
(dissected)Tj
3.7814 0 Td
(according)Tj
4.1329 0 Td
(the)Tj
1.4513 0 Td
(Paxinos)Tj
3.3222 0 Td
(coordinates)Tj
-32.1163 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(]:)Tj
2.0466 0 Td
(hippocampus)Tj
5.5728 0 Td
(\(AP)Tj
/F14 1 Tf
[()]TJ
/F6 1 Tf
(2.12)Tj
3.9742 0 Td
(to)Tj
/F14 1 Tf
1.0148 0 Td
[()]TJ
/F6 1 Tf
(6.0,)Tj
2.16 0 Td
(ML)Tj
1.644 0 Td
(0)Tj
0.686 0 Td
(to)Tj
1.0148 0 Td
(5.0,)Tj
1.5988 0 Td
(DV)Tj
1.6384 0 Td
(5.4)Tj
1.3776 0 Td
(to)Tj
1.0148 0 Td
(7.6\),)Tj
1.9332 0 Td
(amygdala)Tj
3.9855 0 Td
(\(AP)Tj
/F14 1 Tf
1.7858 0 Td
[()]TJ
/F6 1 Tf
(0.58)Tj
2.4094 0 Td
(to)Tj
/F14 1 Tf
1.0148 0 Td
[()]TJ
/F6 1 Tf
(2.18,)Tj
-34.8715 -1.2982 Td
(ML)Tj
1.6384 0 Td
(1.5)Tj
1.3776 0 Td
(to)Tj
1.0148 0 Td
(4.5,)Tj
1.5987 0 Td
(DV)Tj
1.6384 0 Td
(4)Tj
0.686 0 Td
(to)Tj
1.0148 0 Td
(5.75\),)Tj
2.4038 0 Td
(and)Tj
1.7121 0 Td
(frontal)Tj
2.8686 0 Td
(cortex)Tj
2.6759 0 Td
(\(AP)Tj
1.7858 0 Td
(5.20)Tj
1.8482 0 Td
(to)Tj
1.0091 0 Td
(1.70,)Tj
2.0693 0 Td
(ML)Tj
1.6384 0 Td
(0)Tj
0.6917 0 Td
(to)Tj
1.0148 0 Td
(3.3,)Tj
1.5987 0 Td
(DV)Tj
1.6384 0 Td
(9.0)Tj
1.3777 0 Td
(to)Tj
1.0148 0 Td
(4.4\).)Tj
-0.004 Tc
-33.1198 -1.2983 Td
(Total)Tj
2.3187 0 Td
(RNA)Tj
2.262 0 Td
(was)Tj
1.7008 0 Td
(isolated)Tj
3.2825 0 Td
(from)Tj
2.2053 0 Td
(individual)Tj
4.2917 0 Td
(tissue)Tj
2.4548 0 Td
(samples)Tj
3.3845 0 Td
(using)Tj
2.3868 0 Td
(the)Tj
1.474 0 Td
(Direct-zol)Tj
4.2519 0 Td
(RNA)Tj
2.262 0 Td
(MiniPrep)Tj
-33.4712 -1.3039 Td
(Kit)Tj
1.4569 0 Td
(\(Zymo)Tj
2.9424 0 Td
(Research,)Tj
4.0308 0 Td
(Irvine,)Tj
2.8517 0 Td
(CA\).)Tj
2.1316 0 Td
(Briefly,)Tj
3.0784 0 Td
(individual)Tj
4.286 0 Td
(tissue)Tj
2.4604 0 Td
(samples)Tj
3.3846 0 Td
(were)Tj
2.1203 0 Td
(homogenized)Tj
5.6522 0 Td
(in)Tj
1.0318 0 Td
(TRI-)Tj
-35.4271 -1.2983 Td
(zol)Tj
1.3832 0 Td
(reagent)Tj
3.1862 0 Td
(\(Ambion,)Tj
4.1158 0 Td
(Austin,)Tj
3.1351 0 Td
(TX\))Tj
1.8255 0 Td
(and)Tj
1.7292 0 Td
(RNA)Tj
2.262 0 Td
(was)Tj
1.7008 0 Td
(immediately)Tj
5.1987 0 Td
(isolated)Tj
3.2882 0 Td
(using)Tj
2.3867 0 Td
(the)Tj
1.4684 0 Td
(kit)Tj
1.2812 0 Td
(following)Tj
-32.961 -1.2982 Td
(the)Tj
1.4683 0 Td
(manufacturer's)Tj
6.2645 0 Td
(protocol.)Tj
3.8211 0 Td
(Total)Tj
2.3187 0 Td
(RNA)Tj
2.2621 0 Td
(\(2)Tj
/F14 1 Tf
1.0318 0 Td
[()]TJ
/F6 1 Tf
(g\))Tj
1.5363 0 Td
(was)Tj
1.6951 0 Td
(reverse)Tj
3.0501 0 Td
(transcribed)Tj
4.7565 0 Td
(to)Tj
1.0205 0 Td
(generate)Tj
3.6113 0 Td
(double-)Tj
-32.8363 -1.304 Td
(stranded)Tj
3.7133 0 Td
(cDNA)Tj
2.795 0 Td
(using)Tj
2.3924 0 Td
(the)Tj
1.4683 0 Td
(SuperScript)Tj
4.8926 0 Td
(VILO)Tj
2.5341 0 Td
(cDNA)Tj
2.8007 0 Td
(Synthesis)Tj
3.9401 0 Td
(Kit)Tj
1.457 0 Td
(\(ThermoFisher)Tj
6.2985 0 Td
(Scientific,)Tj
-32.292 -1.2982 Td
(Waltham,)Tj
4.1669 0 Td
(MA\).)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 349.1716 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Candidate)Tj
4.6913 0 Td
(quantitativ)Tj
4.4717 0 Td
(e)Tj
0.7654 0 Td
(trait)Tj
1.8567 0 Td
(genes)Tj
2.8984 0 Td
(for)Tj
1.3678 0 Td
(acute)Tj
2.622 0 Td
(stress)Tj
2.8489 0 Td
(responsive)Tj
4.748 0 Td
(ness)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01942)Tj
4.089 0 Td
(93)Tj
0 g
2.0906 0 Td
(March)Tj
2.9622 0 Td
(12,)Tj
1.5874 0 Td
(2018)Tj
34.3562 0 Td
(4)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
100 0 obj
<>stream
endstream
endobj
101 0 obj
<>stream
endstream
endobj
102 0 obj
<>stream
endstream
endobj
103 0 obj
<>stream
endstream
endobj
104 0 obj
<>stream
endstream
endobj
105 0 obj
<>stream
endstream
endobj
106 0 obj
<>stream
BT
66.402 0 Td
(18)Tj
ET
Q
endstream
endobj
107 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 15 0 R/Annots 108 0 R/Contents 109 0 R/TrimBox[0 0 612 792]>>
endobj
108 0 obj
[110 0 R 111 0 R 112 0 R 113 0 R 114 0 R 115 0 R 116 0 R 117 0 R 118 0 R 119 0 R 120 0 R 121 0 R 122 0 R 123 0 R 124 0 R 125 0 R 126 0 R 127 0 R 128 0 R]
endobj
110 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref026)>>
endobj
111 0 obj
<>/Border[0 0 0]/A 129 0 R>>
endobj
129 0 obj
<>
endobj
112 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref026)>>
endobj
113 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref027)>>
endobj
114 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref026)>>
endobj
115 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref028)>>
endobj
116 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref026)>>
endobj
117 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref026)>>
endobj
118 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref029)>>
endobj
119 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref030)>>
endobj
120 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref031)>>
endobj
121 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.s003)>>
endobj
122 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.s005)>>
endobj
123 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref012)>>
endobj
124 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref014)>>
endobj
125 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.ref032)>>
endobj
126 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.t002)>>
endobj
127 0 obj
<>/Border[0 0 0]/Dest(Rpone.0194293.s009)>>
endobj
128 0 obj
<>/Border[0 0 0]/A 130 0 R>>
endobj
130 0 obj
<>
endobj
109 0 obj
[131 0 R 132 0 R 133 0 R 134 0 R 135 0 R 136 0 R 137 0 R 138 0 R 139 0 R 140 0 R 141 0 R 142 0 R 143 0 R 144 0 R 145 0 R 146 0 R 147 0 R]
endobj
131 0 obj
<>stream
q
0.83 0.64 0.02 0 k
453.3732 662.0031 m
462.8976 662.0031 l
h
f*
453.6567 583.9937 m
555.0803 583.9937 l
h
f*
528.8882 571.011 m
538.4126 571.011 l
h
f*
542.8346 571.011 m
552.3591 571.011 l
h
f*
538.8094 558.0283 m
548.3339 558.0283 l
h
f*
552.7559 558.0283 m
562.2803 558.0283 l
h
f*
385.3417 532.0063 m
394.8661 532.0063 l
h
f*
422.589 505.9843 m
432.1134 505.9843 l
h
f*
436.5354 505.9843 m
446.0598 505.9843 l
h
f*
254.778 453.9969 m
264.3024 453.9969 l
h
f*
268.7811 453.9969 m
278.2488 453.9969 l
h
f*
480.6425 389.0268 m
490.1669 389.0268 l
h
f*
495.326 389.0268 m
504.9071 389.0268 l
h
f*
371.1118 319.9748 m
380.6362 319.9748 l
h
f*
385.0583 319.9748 m
394.5827 319.9748 l
h
f*
275.9244 189.978 m
285.4488 189.978 l
h
f*
383.4709 150.9732 m
412.6677 150.9732 l
h
f*
492.3213 150.9732 m
526.2236 150.9732 l
h
f*
0 g
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 706.0535 cm
BT
/F11 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Microarray)Tj
4.9323 0 Td
(analysis)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 689.1023 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Genome-wide)Tj
5.9073 0 Td
(microarray)Tj
4.6772 0 Td
(analysis)Tj
3.3278 0 Td
(was)Tj
1.6951 0 Td
(performed)Tj
4.4731 0 Td
(from)Tj
2.2053 0 Td
(RNA)Tj
2.262 0 Td
(isolated)Tj
3.2825 0 Td
(from)Tj
2.2054 0 Td
(hippocampi,)Tj
-30.0357 -1.3039 Td
(amygdalae,)Tj
4.6828 0 Td
(and)Tj
1.7291 0 Td
(frontal)Tj
2.914 0 Td
(cortexes)Tj
3.4922 0 Td
(of)Tj
1.0148 0 Td
(F344)Tj
2.177 0 Td
(and)Tj
1.7292 0 Td
(WKY)Tj
2.4944 0 Td
(adult)Tj
2.2507 0 Td
(male)Tj
2.143 0 Td
(rats)Tj
1.6838 0 Td
(that)Tj
1.7801 0 Td
(were)Tj
2.1203 0 Td
(unstressed,)Tj
4.6488 0 Td
(with-)Tj
-34.8602 -1.2982 Td
(out)Tj
1.5533 0 Td
(undergoing)Tj
4.8699 0 Td
(any)Tj
1.6554 0 Td
(behavioral)Tj
4.3937 0 Td
(testing,)Tj
3.1068 0 Td
(as)Tj
1.0148 0 Td
(described)Tj
4.0535 0 Td
(previously)Tj
4.3483 0 Td
([)Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
(].)Tj
2.0806 0 Td
(Briefly,)Tj
3.0728 0 Td
(the)Tj
1.474 0 Td
(cDNA)Tj
2.8006 0 Td
(gener-)Tj
-34.4237 -1.2983 Td
(ated)Tj
1.9048 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.4683 0 Td
(RNA)Tj
2.262 0 Td
(was)Tj
1.7008 0 Td
(linearly)Tj
3.2088 0 Td
(amplified)Tj
4.0082 0 Td
(and)Tj
1.7348 0 Td
(labeled)Tj
3.0273 0 Td
(with)Tj
1.9956 0 Td
(biotinylated)Tj
5.0003 0 Td
(nucleotides)Tj
4.7735 0 Td
(in)Tj
1.0375 0 Td
(an)Tj
/F10 1 Tf
1.2019 0 Td
[(\r)12()]TJ
-35.5292 -1.3039 Td
[()-13(\r)14()7()20()]TJ
/F6 1 Tf
2.0466 0 Td
(transcription)Tj
5.4311 0 Td
(reaction)Tj
3.4923 0 Td
(using)Tj
2.3924 0 Td
(the)Tj
1.4683 0 Td
(Illumina)Tj
3.651 0 Td
(TotalPrep)Tj
4.1839 0 Td
(RNA)Tj
2.2621 0 Td
(Amplification)Tj
5.7883 0 Td
(Kit)Tj
1.4626 0 Td
(\(San)Tj
2.0183 0 Td
(Diego,)Tj
-34.1969 -1.2983 Td
(CA\))Tj
1.9048 0 Td
(to)Tj
1.0262 0 Td
(make)Tj
2.3924 0 Td
(cRNA.)Tj
2.914 0 Td
(1.5)Tj
/F14 1 Tf
1.3946 0 Td
[()]TJ
/F6 1 Tf
(g)Tj
1.1906 0 Td
(of)Tj
1.0148 0 Td
(biotin-labelled)Tj
6.0207 0 Td
(and)Tj
1.7291 0 Td
(fragmented)Tj
4.8246 0 Td
(cRNA)Tj
2.6815 0 Td
(was)Tj
1.7008 0 Td
(then)Tj
2.0183 0 Td
(hybridized)Tj
4.507 0 Td
(onto)Tj
-35.3194 -1.2982 Td
(RatRef-12)Tj
4.2065 0 Td
(Expression)Tj
4.6148 0 Td
(BeadChips)Tj
4.5184 0 Td
(\(Illumina,)Tj
4.2179 0 Td
(San)Tj
1.6781 0 Td
(Diego,)Tj
2.8403 0 Td
(CA\).)Tj
2.1317 0 Td
(The)Tj
1.7858 0 Td
(BeadChips)Tj
4.5184 0 Td
(have)Tj
2.0636 0 Td
(multiple)Tj
-32.5755 -1.304 Td
(probes)Tj
2.9083 0 Td
(per)Tj
1.5307 0 Td
(transcript)Tj
4.1102 0 Td
(to)Tj
1.0261 0 Td
(mitigate)Tj
3.4923 0 Td
(hybridization)Tj
5.6125 0 Td
(bias)Tj
1.7859 0 Td
(artifacts.)Tj
3.6226 0 Td
(Probe)Tj
2.5852 0 Td
(intensity)Tj
3.6907 0 Td
(data)Tj
1.9105 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
-34.4804 -1.2982 Td
(BeadChips)Tj
4.5183 0 Td
(were)Tj
2.126 0 Td
(directly)Tj
3.2315 0 Td
(read)Tj
1.9786 0 Td
(into)Tj
1.8368 0 Td
(the)Tj
1.4683 0 Td
(R)Tj
0.8448 0 Td
(software)Tj
3.5886 0 Td
(environment)Tj
5.4311 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(http://www.R-project.org)Tj
0 g
(\))Tj
-25.024 -1.2983 Td
(from)Tj
2.2053 0 Td
(bead)Tj
2.109 0 Td
(summary)Tj
4.0081 0 Td
(files)Tj
1.8085 0 Td
(produced)Tj
4.0479 0 Td
(by)Tj
1.1792 0 Td
(BeadStudio)Tj
4.7905 0 Td
(using)Tj
2.3867 0 Td
(the)Tj
1.4684 0 Td
(R/beadarray)Tj
5.1307 0 Td
(package)Tj
3.4128 0 Td
([)Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(27)Tj
0 g
(].)Tj
-34.2819 -1.2983 Td
(Quantile)Tj
3.7133 0 Td
(normalization)Tj
5.879 0 Td
(was)Tj
1.7008 0 Td
(applied)Tj
3.1521 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(data)Tj
1.9162 0 Td
(using)Tj
2.3868 0 Td
(the)Tj
1.4683 0 Td
(R/preprocessCore)Tj
7.4154 0 Td
(package)Tj
3.4129 0 Td
([)Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(28)Tj
0 g
(].)Tj
-35.2741 -1.3039 Td
(Data)Tj
2.1203 0 Td
(quality)Tj
2.9536 0 Td
(was)Tj
1.7008 0 Td
(assessed)Tj
3.4753 0 Td
(using)Tj
2.3867 0 Td
(histograms)Tj
4.6318 0 Td
(of)Tj
1.0148 0 Td
(signal)Tj
2.5455 0 Td
(intensities,)Tj
4.5071 0 Td
(scatter)Tj
2.8289 0 Td
(plots,)Tj
2.3754 0 Td
(and)Tj
1.7292 0 Td
(hierarchical)Tj
-32.2694 -1.2982 Td
(clustering)Tj
4.1498 0 Td
(of)Tj
1.0148 0 Td
(samples,)Tj
3.6114 0 Td
(as)Tj
1.0148 0 Td
(previously)Tj
4.3483 0 Td
(described)Tj
4.0535 0 Td
([)Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
(].)Tj
2.0749 0 Td
(Statistical)Tj
4.0252 0 Td
(significance)Tj
4.9379 0 Td
(of)Tj
1.0148 0 Td
(microarray)Tj
-30.2454 -1.2983 Td
(expression)Tj
4.4617 0 Td
(differences)Tj
4.5694 0 Td
(between)Tj
3.5263 0 Td
(F344)Tj
2.1769 0 Td
(and)Tj
1.7292 0 Td
(WKY)Tj
2.4888 0 Td
(was)Tj
1.7007 0 Td
(determined)Tj
4.8529 0 Td
(using)Tj
2.3868 0 Td
(ANOVA)Tj
3.7644 0 Td
(methods)Tj
-31.6571 -1.3039 Td
(within)Tj
2.8119 0 Td
(the)Tj
1.4683 0 Td
(R/maanova)Tj
4.8019 0 Td
(package)Tj
3.4186 0 Td
(as)Tj
1.0148 0 Td
(previously)Tj
4.3483 0 Td
(described)Tj
4.0535 0 Td
([)Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(29)Tj
0 g
(].)Tj
1.7348 0 Td
(DEGs)Tj
2.6022 0 Td
(were)Tj
2.1259 0 Td
(determined)Tj
-30.115 -1.2983 Td
(between)Tj
3.5262 0 Td
(strains)Tj
2.863 0 Td
(with)Tj
1.9956 0 Td
(an)Tj
1.2019 0 Td
(FDR-adjusted)Tj
/F10 1 Tf
5.8166 0 Td
[()]TJ
/F6 1 Tf
(value)Tj
3.3676 0 Td
(less)Tj
1.6157 0 Td
(than)Tj
2.0296 0 Td
(0.05)Tj
1.8708 0 Td
(and)Tj
1.7292 0 Td
(a)Tj
0.6519 0 Td
(fold)Tj
1.7915 0 Td
(change)Tj
3.0387 0 Td
(greater)Tj
3.0104 0 Td
(than)Tj
-34.5087 -1.2982 Td
(1.3)Tj
1.3946 0 Td
(\(30%)Tj
2.2677 0 Td
(increase)Tj
3.4752 0 Td
(or)Tj
1.0942 0 Td
(decrease\).)Tj
4.1783 0 Td
(This)Tj
1.9842 0 Td
(criterion)Tj
3.6963 0 Td
(has)Tj
1.5421 0 Td
(been)Tj
2.126 0 Td
(well-established)Tj
6.5479 0 Td
(to)Tj
1.0262 0 Td
(give)Tj
1.8255 0 Td
(biologically)Tj
-31.1582 -1.304 Td
(meaningful)Tj
4.7848 0 Td
(datasets)Tj
3.3619 0 Td
(when)Tj
2.4037 0 Td
(interpreting)Tj
5.0173 0 Td
(differential)Tj
4.6035 0 Td
(gene)Tj
2.0806 0 Td
(expression)Tj
4.4674 0 Td
(profiles)Tj
3.2031 0 Td
(in)Tj
1.0318 0 Td
(microarray)Tj
-30.9541 -1.2982 Td
(experiments)Tj
5.1363 0 Td
([)Tj
0.83 0.64 0.02 0 k
(30)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7405 0 Td
(31)Tj
0 g
(].)Tj
1.7347 0 Td
(The)Tj
1.7802 0 Td
(microarray)Tj
4.6771 0 Td
(data)Tj
1.9106 0 Td
(used)Tj
2.0636 0 Td
(the)Tj
1.4683 0 Td
(rat)Tj
1.321 0 Td
(genome)Tj
3.4072 0 Td
(RGSC_v3.4)Tj
4.8245 0 Td
(for)Tj
1.389 0 Td
(identification)Tj
-31.453 -1.2983 Td
(of)Tj
1.0147 0 Td
(transcripts,)Tj
4.6942 0 Td
(which)Tj
2.6532 0 Td
(was)Tj
1.6951 0 Td
(translated)Tj
4.1669 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(latest)Tj
2.279 0 Td
(version)Tj
3.1521 0 Td
(of)Tj
1.0148 0 Td
(rat)Tj
1.3266 0 Td
(genome,)Tj
3.634 0 Td
(Rnor_6.0.)Tj
-26.9289 -1.2983 Td
(The)Tj
1.7858 0 Td
(differential)Tj
4.6034 0 Td
(gene)Tj
2.0806 0 Td
(expression)Tj
4.4617 0 Td
(profiles)Tj
3.2032 0 Td
(between)Tj
3.5319 0 Td
(the)Tj
1.4683 0 Td
(two)Tj
1.7065 0 Td
(strains)Tj
2.863 0 Td
(were)Tj
2.1203 0 Td
(determined)Tj
4.8529 0 Td
(with)Tj
1.9955 0 Td
(a)Tj
-35.8693 -1.3039 Td
(significance)Tj
4.9379 0 Td
(criteria)Tj
3.0727 0 Td
(of)Tj
1.0148 0 Td
(an)Tj
/F10 1 Tf
1.2019 0 Td
[()13()]TJ
/F6 1 Tf
(-adjusted)Tj
/F10 1 Tf
5.7316 0 Td
[()]TJ
/F6 1 Tf
0.771 0 Td
(value)Tj
2.3017 0 Td
(less)Tj
1.6158 0 Td
(than)Tj
2.0239 0 Td
(0.05)Tj
1.8765 0 Td
(and)Tj
1.7291 0 Td
(an)Tj
1.2019 0 Td
(absolute)Tj
3.5263 0 Td
(fold)Tj
1.7858 0 Td
(change)Tj
-32.7909 -1.2982 Td
(above)Tj
2.5454 0 Td
(1.3)Tj
1.3947 0 Td
(\(30%)Tj
2.2677 0 Td
(increase)Tj
3.4752 0 Td
(or)Tj
1.0999 0 Td
(decrease\).)Tj
4.1725 0 Td
(With)Tj
2.2791 0 Td
(these)Tj
2.2507 0 Td
(criteria,)Tj
3.3052 0 Td
(we)Tj
1.3266 0 Td
(found)Tj
2.6192 0 Td
(1,030)Tj
2.347 0 Td
(DEGs)Tj
2.6022 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(hippo-)Tj
-34.1912 -1.2983 Td
(campus,)Tj
3.5262 0 Td
(769)Tj
1.6498 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(amygdala,)Tj
4.2576 0 Td
(and)Tj
1.7291 0 Td
(976)Tj
1.6498 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(frontal)Tj
2.914 0 Td
(cortex,)Tj
2.931 0 Td
(as)Tj
1.0148 0 Td
(listed)Tj
2.3471 0 Td
(in)Tj
0.83 0.64 0.02 0 k
1.0318 0 Td
(S3)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(S5)Tj
0 g
2.6418 0 Td
(Tables.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 364.0251 cm
BT
/F11 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Identification)Tj
5.9669 0 Td
(of)Tj
1.0678 0 Td
(single)Tj
2.6456 0 Td
(nucleotide)Tj
4.5922 0 Td
(polymorphisms)Tj
6.7749 0 Td
(in)Tj
1.0677 0 Td
(coding)Tj
3.0378 0 Td
(regions)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 347.0173 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(The)Tj
1.7801 0 Td
(genes)Tj
2.4434 0 Td
(with)Tj
1.9956 0 Td
(non-synonymous)Tj
7.268 0 Td
(single)Tj
2.5285 0 Td
(nucleotide)Tj
4.4163 0 Td
(polymorphisms)Tj
6.497 0 Td
(\(SNPs\))Tj
3.0274 0 Td
(within)Tj
2.8176 0 Td
(coding)Tj
-32.7739 -1.2982 Td
(regions)Tj
3.1521 0 Td
(between)Tj
3.5206 0 Td
(the)Tj
1.4626 0 Td
(F344)Tj
2.1657 0 Td
(and)Tj
1.7234 0 Td
(WKY)Tj
2.4832 0 Td
(genomes)Tj
3.7644 0 Td
(were)Tj
2.1146 0 Td
(obtained)Tj
3.7247 0 Td
(from)Tj
2.194 0 Td
(the)Tj
1.4627 0 Td
(Rat)Tj
1.5647 0 Td
(Genome)Tj
3.651 0 Td
(Sequencing)Tj
-32.9837 -1.2983 Td
(and)Tj
1.7291 0 Td
(Mapping)Tj
3.8607 0 Td
(Consortium)Tj
5.091 0 Td
(and)Tj
1.7292 0 Td
(Baud)Tj
2.2903 0 Td
(et)Tj
0.9411 0 Td
(al.)Tj
1.1282 0 Td
([)Tj
0.83 0.64 0.02 0 k
(12)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(14)Tj
0 g
(].)Tj
1.7405 0 Td
(In)Tj
1.1055 0 Td
(these)Tj
2.2563 0 Td
(studies,)Tj
3.2088 0 Td
(both)Tj
2.0636 0 Td
(F344)Tj
2.1714 0 Td
(and)Tj
1.7291 0 Td
(WKY)Tj
-32.7796 -1.3039 Td
(genomes)Tj
3.7643 0 Td
(were)Tj
2.126 0 Td
(first)Tj
1.8028 0 Td
(mapped)Tj
3.4639 0 Td
(to)Tj
1.0205 0 Td
(the)Tj
1.474 0 Td
(Brown)Tj
2.9083 0 Td
(Norway)Tj
3.4073 0 Td
(reference)Tj
3.9174 0 Td
(genome,)Tj
3.634 0 Td
(version)Tj
3.1521 0 Td
(RGSC_3.4.)Tj
-30.6706 -1.2983 Td
(Using)Tj
2.5908 0 Td
(the)Tj
1.4683 0 Td
(Integrative)Tj
4.5411 0 Td
(Genomics)Tj
4.286 0 Td
(Viewer)Tj
3.0784 0 Td
(\(Broad)Tj
2.9763 0 Td
(Institute,)Tj
3.8041 0 Td
(Cambridge,)Tj
4.9209 0 Td
(Massachusetts\),)Tj
6.4856 0 Td
(we)Tj
1.3267 0 Td
(iden-)Tj
-35.4782 -1.2982 Td
(tified)Tj
2.2903 0 Td
(the)Tj
1.4684 0 Td
(coding)Tj
2.9593 0 Td
(sequence)Tj
3.8608 0 Td
(variations)Tj
4.1612 0 Td
(between)Tj
3.5263 0 Td
(the)Tj
1.474 0 Td
(F344)Tj
2.1713 0 Td
(and)Tj
1.7291 0 Td
(WKY)Tj
2.4945 0 Td
(genomes)Tj
3.7701 0 Td
(and)Tj
1.7291 0 Td
(translated)Tj
-31.6344 -1.2983 Td
(them)Tj
2.279 0 Td
(to)Tj
1.0205 0 Td
(the)Tj
1.4626 0 Td
(rat)Tj
1.3153 0 Td
(genome)Tj
3.3959 0 Td
(version)Tj
3.1464 0 Td
(Rnor_6.0.)Tj
4.1612 0 Td
(For)Tj
1.6158 0 Td
(quality)Tj
2.948 0 Td
(control,)Tj
3.3392 0 Td
(we)Tj
1.3209 0 Td
(set)Tj
1.2983 0 Td
(the)Tj
1.457 0 Td
(criteria)Tj
3.0727 0 Td
(that)Tj
1.7688 0 Td
(the)Tj
1.4627 0 Td
(cover-)Tj
-35.0643 -1.3039 Td
(age)Tj
1.542 0 Td
(for)Tj
1.3833 0 Td
(each)Tj
2.0296 0 Td
(SNPs)Tj
2.3527 0 Td
(had)Tj
1.7121 0 Td
(to)Tj
1.0205 0 Td
(be)Tj
1.1509 0 Td
(greater)Tj
3.0103 0 Td
(than)Tj
2.0296 0 Td
(10)Tj
1.1679 0 Td
(reads,)Tj
2.5625 0 Td
(where)Tj
2.6532 0 Td
(reads)Tj
2.3357 0 Td
(are)Tj
1.4514 0 Td
(used)Tj
2.0579 0 Td
(to)Tj
1.0261 0 Td
(reconstruct)Tj
4.7736 0 Td
(the)Tj
-34.2593 -1.2983 Td
(sequence.)Tj
4.0818 0 Td
(The)Tj
1.7802 0 Td
(more)Tj
2.3414 0 Td
(reads)Tj
2.3414 0 Td
(a)Tj
0.6519 0 Td
(sequence)Tj
3.8608 0 Td
(had,)Tj
1.9332 0 Td
(the)Tj
1.4683 0 Td
(more)Tj
2.3414 0 Td
(reliable)Tj
3.1408 0 Td
(the)Tj
1.4684 0 Td
(data.)Tj
2.1373 0 Td
(Furthermore,)Tj
5.6182 0 Td
(the)Tj
1.4683 0 Td
(single)Tj
-34.6334 -1.2982 Td
(nucleotide)Tj
4.4106 0 Td
(variations)Tj
4.1669 0 Td
(had)Tj
1.7065 0 Td
(to)Tj
1.0261 0 Td
(be)Tj
1.1509 0 Td
(called)Tj
2.5228 0 Td
(in)Tj
1.0318 0 Td
(at)Tj
0.9524 0 Td
(least)Tj
1.9843 0 Td
(50%)Tj
1.9275 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(reads.)Tj
-22.1668 -1.304 Td
(Among)Tj
3.2484 0 Td
(inherited)Tj
3.8721 0 Td
(gene)Tj
2.0807 0 Td
(variations)Tj
4.1669 0 Td
(in)Tj
1.0318 0 Td
(humans,)Tj
3.668 0 Td
(nonsynonymous)Tj
6.9108 0 Td
(single)Tj
2.5342 0 Td
(nucleotide)Tj
4.4106 0 Td
(polymor-)Tj
-33.1197 -1.2982 Td
(phisms)Tj
3.0727 0 Td
(that)Tj
1.7801 0 Td
(lead)Tj
1.8482 0 Td
(to)Tj
1.0261 0 Td
(an)Tj
1.2019 0 Td
(amino)Tj
2.795 0 Td
(acid)Tj
1.8765 0 Td
(change)Tj
3.0387 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(protein)Tj
3.1521 0 Td
(product)Tj
3.3959 0 Td
(are)Tj
1.4457 0 Td
(most)Tj
2.211 0 Td
(relevant)Tj
3.4185 0 Td
(to)Tj
1.0262 0 Td
(human)Tj
-33.7944 -1.2983 Td
(inherited)Tj
3.8721 0 Td
(diseases)Tj
3.3788 0 Td
([)Tj
0.83 0.64 0.02 0 k
(32)Tj
0 g
(].)Tj
2.0806 0 Td
(Therefore,)Tj
4.388 0 Td
(as)Tj
1.0148 0 Td
(the)Tj
1.4741 0 Td
(first)Tj
1.8028 0 Td
(step)Tj
1.8198 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(identification)Tj
5.5842 0 Td
(of)Tj
1.0148 0 Td
(candidate)Tj
4.1046 0 Td
(QTGs,)Tj
2.88 0 Td
(we)Tj
-35.9204 -1.3039 Td
(focused)Tj
3.2768 0 Td
(on)Tj
1.2756 0 Td
(this)Tj
1.6724 0 Td
(subset)Tj
2.6986 0 Td
(of)Tj
1.0148 0 Td
(genes.)Tj
2.6702 0 Td
(Genes)Tj
2.6929 0 Td
(with)Tj
1.9955 0 Td
(non-synonymous)Tj
7.2624 0 Td
(SNPs)Tj
2.3527 0 Td
(within)Tj
2.812 0 Td
(the)Tj
1.474 0 Td
(heterozygous)Tj
/F10 1 Tf
-31.1979 -1.2983 Td
[()10()7()20()8()10()]TJ
/F6 1 Tf
3.4582 0 Td
(region)Tj
2.8063 0 Td
(were)Tj
2.1203 0 Td
(excluded)Tj
3.787 0 Td
(from)Tj
2.2054 0 Td
(our)Tj
1.6271 0 Td
(analysis.)Tj
3.5489 0 Td
(Of)Tj
1.2586 0 Td
(the)Tj
1.4683 0 Td
(16)Tj
1.1679 0 Td
(DEGs)Tj
2.6022 0 Td
(mapped)Tj
3.4639 0 Td
(within)Tj
2.8119 0 Td
(the)Tj
/F10 1 Tf
1.4684 0 Td
[()10()7()20()8()10()]TJ
/F6 1 Tf
-33.7944 -1.2982 Td
(region,)Tj
3.0273 0 Td
(10)Tj
1.1679 0 Td
(genes)Tj
2.4434 0 Td
(were)Tj
2.126 0 Td
(found)Tj
2.6192 0 Td
(to)Tj
1.0205 0 Td
(contain)Tj
3.2485 0 Td
(SNPs)Tj
2.3527 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Table)Tj
2.7836 0 Td
(2)Tj
0 g
(\).)Tj
1.2586 0 Td
(SNPs)Tj
2.3527 0 Td
(are)Tj
1.4457 0 Td
(listed)Tj
2.3471 0 Td
(in)Tj
0.83 0.64 0.02 0 k
1.0374 0 Td
(S9)Tj
1.1679 0 Td
(Table)Tj
0 g
(.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 125.9716 cm
BT
/F11 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Real-time)Tj
4.2897 0 Td
(RT-PCR)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 109.0204 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(The)Tj
1.7801 0 Td
(real-time)Tj
3.8551 0 Td
(reverse)Tj
3.05 0 Td
(transcription-polymerase)Tj
10.3408 0 Td
(chain)Tj
2.4207 0 Td
(reaction)Tj
3.4923 0 Td
(\(qRT-PCR\))Tj
4.8359 0 Td
(was)Tj
1.7008 0 Td
(carried)Tj
3.0387 0 Td
(out)Tj
-34.5144 -1.3039 Td
(using)Tj
2.3867 0 Td
(RNA)Tj
2.262 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.474 0 Td
(second)Tj
3.0104 0 Td
(cohort)Tj
2.8516 0 Td
(of)Tj
1.0148 0 Td
(F344)Tj
2.177 0 Td
(and)Tj
1.7291 0 Td
(WKY)Tj
2.4945 0 Td
(rats)Tj
1.6838 0 Td
(that)Tj
1.7744 0 Td
(were)Tj
2.126 0 Td
(subjected)Tj
3.9685 0 Td
(to)Tj
1.0261 0 Td
(CFC)Tj
2.075 0 Td
(testing,)Tj
-34.2593 -1.2982 Td
(as)Tj
1.0147 0 Td
(well)Tj
1.8199 0 Td
(as)Tj
1.0148 0 Td
(RNA)Tj
2.262 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
1.4684 0 Td
(F344.WKY-)Tj
/F10 1 Tf
[()10()7()20()8()10()]TJ
/F6 1 Tf
8.2658 0 Td
(rats.)Tj
1.9105 0 Td
(Primers)Tj
3.3902 0 Td
(for)Tj
1.389 0 Td
(each)Tj
2.0296 0 Td
(gene)Tj
2.0806 0 Td
(were)Tj
2.1203 0 Td
(designed)Tj
3.7644 0 Td
(using)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 349.1716 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Candidate)Tj
4.6913 0 Td
(quantitativ)Tj
4.4717 0 Td
(e)Tj
0.7654 0 Td
(trait)Tj
1.8567 0 Td
(genes)Tj
2.8984 0 Td
(for)Tj
1.3678 0 Td
(acute)Tj
2.622 0 Td
(stress)Tj
2.8489 0 Td
(responsive)Tj
4.748 0 Td
(ness)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01942)Tj
4.089 0 Td
(93)Tj
0 g
2.0906 0 Td
(March)Tj
2.9622 0 Td
(12,)Tj
1.5874 0 Td
(2018)Tj
34.3562 0 Td
(5)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
132 0 obj
<>stream
endstream
endobj
133 0 obj
<>stream
endstream
endobj
134 0 obj
<>stream
endstream
endobj
135 0 obj
<>stream
endstream
endobj
136 0 obj
<>stream
endstream
endobj
137 0 obj
<>stream
endstream
endobj
138 0 obj
<>stream
endstream
endobj
139 0 obj
<>stream
endstream
endobj
140 0 obj
<>stream
endstream
endobj
141 0 obj
<>stream
endstream
endobj
142 0 obj
<>stream
endstream
endobj
143 0 obj
<>stream
endstream
endobj
144 0 obj
<>stream
endstream
endobj
145 0 obj
<>stream
endstream
endobj
146 0 obj
<>stream
endstream
endobj
147 0 obj
<>stream
BT
66.402 0 Td
(18)Tj
ET
Q
endstream
endobj
148 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 15 0 R/Annots 151 0 R/Contents 152 0 R/TrimBox[0 0 612 792]>>
endobj
149 0 obj
<>
endobj
153 0 obj
<>stream
/CIDInit/ProcSet findresource begin
12 dict begin begincmap
/CMapName/Adobe-Identity-UCS def
/CIDSystemInfo<>def
/CMapType 2 def
1 begincodespacerange
<0000>
endcodespacerange
1 beginbfchar
<00EF><0394>
endbfchar
endcmap
CMapName currentdict /CMap defineresource pop end end
endstream
endobj
154 0 obj
<>/FontDescriptor 155 0 R/DW 250/W[239[603]]>>
endobj
155 0 obj
<>
endobj
156 0 obj
<>stream
cvt f6 fpgm2Msf bglyfcW Dhead= 6hhea1 $hmtx&O