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Alternate splicing of mouse fusin/CXC chemokine receptor-4: stromal cell-derived factor-1α is a ligand for both CXC chemokine receptor-4 isoforms

Item Type:Article
Title:Alternate splicing of mouse fusin/CXC chemokine receptor-4: stromal cell-derived factor-1α is a ligand for both CXC chemokine receptor-4 isoforms
Creators Name:Heesen, M. and Berman, M.A. and Hoepken, U.E. and Gerard, N.P. and Dorf, M.E.
Abstract:Inspection of the intron splice junction of the mouse chemokine receptor fusin/CXC chemokine receptor R-4 (CXCR-4) revealed a potential in-frame alternative splice site in the region encoding the N-terminal ectodomain of the receptor. Both predicted splice products were detected by reverse transcriptase-PCR. Cell lines of T lymphocyte, B lymphocyte, and macrophage lineage, plus populations of elicited peritoneal exudate cells, thymocytes, and astrocytes coexpressed mRNA for both isoforms. The full length cDNA of the shorter alternate splice product, termed CXCR-4B, was cloned from peritoneal exudate cell RNA. Chinese hamster ovary cells transfected with either isoform, CXCR-4A or CXCR-4B, responded to stromal cell-derived factor-1{alpha} with a rise in intracellular calcium. Both alternate splice products are therefore functional stromal cell-derived factor-1 {alpha} receptors.
Keywords:Alternative Splicing, Chemokine CXCL12, Chemokines, CXC Chemokines, CXCR4 Receptors, Gene Expression Regulation, HIV Receptors, Ligands, Membrane Proteins, Molecular Sequence Data, Animals, Cricetinae, Mice
Source:Journal of Immunology
ISSN:0022-1767
Publisher:American Association of Immunologists (U.S.A.)
Volume:158
Number:8
Page Range:3561-3564
Date:15 April 1997
Official Publication:http://www.jimmunol.org/content/158/8/3561.abstract
PubMed:View item in PubMed

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