![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
| Item Type: | Article |
|---|---|
| Title: | Modeling cardiovascular diseases with patient-specific human pluripotent stem cell-derived cardiomyocytes |
| Creators Name: | Burridge, P.W., Diecke, S., Matsa, E., Sharma, A., Wu, H. and Wu, J.C. |
| Abstract: | The generation of cardiomyocytes from human induced pluripotent stem cells (hiPSCs) provides a source of cells that accurately recapitulate the human cardiac pathophysiology. The application of these cells allows for modeling of cardiovascular diseases, providing a novel understanding of human disease mechanisms and assessment of therapies. Here, we describe a stepwise protocol developed in our laboratory for the generation of hiPSCs from patients with a specific disease phenotype, long-term hiPSC culture and cryopreservation, differentiation of hiPSCs to cardiomyocytes, and assessment of disease phenotypes. Our protocol combines a number of innovative tools that include a codon-optimized mini intronic plasmid (CoMiP), chemically defined culture conditions to achieve high efficiencies of reprogramming and differentiation, and calcium imaging for assessment of cardiomyocyte phenotypes. Thus, this protocol provides a complete guide to use a patient cohort on a testable cardiomyocyte platform for pharmacological drug assessment. |
| Keywords: | Cardiomyocytes, Disease Modeling, Human Induced Pluripotent Stem Cells, Calcium Imaging |
| Source: | Methods in Molecular Biology |
| Series Name: | Methods in Molecular Biology |
| Title of Book: | Patient-Specific Induced Pluripotent Stem Cell Models |
| ISSN: | 1064-3745 |
| ISBN: | 978-1-4939-3033-3 |
| Publisher: | Springer / Humana Press |
| Volume: | 1353 |
| Page Range: | 119-130 |
| Date: | 2016 |
| Additional Information: | Copyright © 2015 Springer Science+Business Media New York |
| Official Publication: | https://doi.org/10.1007/7651_2015_196 |
| External Fulltext: | View full text on PubMed Central |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
