Isolation of human colon stem cells using surface expression of PTK7

Jung, P.; Sommer, C.; Barriga, F.M.; Buczacki, S.J.; Hernando-Momblona, X.; Sevillano, M.; Duran-Frigola, M.; Aloy, P.; Selbach, M.; Winton, D.J.; Batlle, E.

Isolation of human colon stem cells using surface expression of PTK7

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Item Type:	Article
Title:	Isolation of human colon stem cells using surface expression of PTK7
Creators Name:	Jung, P., Sommer, C., Barriga, F.M., Buczacki, S.J., Hernando-Momblona, X., Sevillano, M., Duran-Frigola, M., Aloy, P., Selbach, M., Winton, D.J. and Batlle, E.
Abstract:	Insertion of reporter cassettes into the Lgr5 locus has enabled the characterization of mouse intestinal stem cells (ISCs). However, low cell surface abundance of LGR5 protein and lack of high-affinity anti-LGR5 antibodies represent a roadblock to efficiently isolate human colonic stem cells (hCoSCs). We set out to identify stem cell markers that would allow for purification of hCoSCs. In an unbiased approach, membrane-enriched protein fractions derived from in vitro human colonic organoids were analyzed by quantitative mass spectrometry. Protein tyrosine pseudokinase PTK7 specified a cell population within human colonic organoids characterized by highest self-renewal and re-seeding capacity. Antibodies recognizing the extracellular domain of PTK7 allowed us to isolate and expand hCoSCs directly from patient-derived mucosa samples. Human PTK7+ cells display features of canonical Lgr5+ ISCs and include a fraction of cells that undergo differentiation toward enteroendocrine lineage that resemble crypt label retaining cells (LRCs).
Keywords:	Cell Adhesion Molecules, Cell Proliferation, Cell Separation, Colon, Cultured Cells, Mass Spectrometry, Organ Culture Techniques, Receptor Protein-Tyrosine Kinases, Stem Cells
Source:	Stem Cell Reports
ISSN:	2213-6711
Publisher:	Cell Press / Elsevier
Volume:	5
Number:	6
Page Range:	979-987
Date:	8 December 2015
Official Publication:	https://doi.org/10.1016/j.stemcr.2015.10.003
PubMed:	View item in PubMed

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