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Successful replication of GWAS hits for multiple sclerosis in 10,000 Germans using the exome array

Item Type:Article
Title:Successful replication of GWAS hits for multiple sclerosis in 10,000 Germans using the exome array
Creators Name:Dankowski, T. and Buck, D. and Andlauer, T.F.M. and Antony, G. and Bayas, A. and Bechmann, L. and Berthele, A. and Bettecken, T. and Chan, A. and Franke, A. and Gold, R. and Graetz, C. and Haas, J. and Hecker, M. and Herms, S. and Infante-Duarte, C. and Jöckel, K.H. and Kieseier, B.C. and Knier, B. and Knop, M. and Kümpfel, T. and Lichtner, P. and Lieb, W. and Lill, C.M. and Limmroth, V. and Linker, R.A. and Loleit, V. and Meuth, S.G. and Moebus, S. and Müller-Myhsok, B. and Nischwitz, S. and Nöthen, M.M. and Paul, F. and Puetz, M. and Ruck, T. and Salmen, A. and Stangel, M. and Stellmann, J.P. and Strauch, K. and Stürner, K.H. and Tackenberg, B. and Then Bergh, F. and Tumani, H. and Waldenberger, M. and Weber, F. and Wiendl, H. and Wildemann, B. and Zettl, U.K. and Ziemann, U. and Zipp, F. and Hemmer, B. and Ziegler, A.
Abstract:Genome-wide association studies (GWAS) successfully identified various chromosomal regions to be associated with multiple sclerosis (MS). The primary aim of this study was to replicate reported associations from GWAS using an exome array in a large German study. German MS cases (n = 4,476) and German controls (n = 5,714) were genotyped using the Illumina HumanExome v1-Chip. Genotype calling was performed with the Illumina Genome Studio(TM) Genotyping Module, followed by zCall. Single-nucleotide polymorphisms (SNPs) in seven regions outside the human leukocyte antigen (HLA) region showed genome-wide significant associations with MS (P values < 5 × 10(-8)). These associations have been reported previously. In addition, SNPs in three previously reported regions outside the HLA region yielded P values < 10(-5) . The effect of nine SNPs in the HLA region remained (P < 10(-5)) after adjustment for other significant SNPs in the HLA region. All of these findings have been reported before or are driven by known risk loci. In summary, findings from previous GWAS for MS could be successfully replicated. We conclude that the regions identified in previous GWAS are also associated in the German population. This reassures the need for detailed investigations of the functional mechanisms underlying the replicated associations.
Keywords:Genome-Wide Association Study, Illumina Exome Array, MMEL1 Gene, ANKRD55 Gene, HLA-DPB2 Gene, Multiple Sclerosis
Source:Genetic Epidemiology
Page Range:601-608
Date:December 2015
Official Publication:https://doi.org/10.1002/gepi.21933
PubMed:View item in PubMed

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