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Imaging protein interactions by FRET microscopy: labeling proteins with fluorescent dyes

Item Type:Article
Title:Imaging protein interactions by FRET microscopy: labeling proteins with fluorescent dyes
Creators Name:Verveer, P.J., Rocks, O., Harpur, A.G. and Bastiaens, P.I.H.
Abstract:This protocol provides a method for labeling proteins, such as antibodies and purified recombinant proteins, with succinimide esters of sulfoindocyanine (Cy) dyes. Cy dyes covalently bind to free amino groups (the {alpha}-amino-terminal or {epsilon}-amino groups on lysine side chains).
Source:Cold Spring Harbor Protocols
ISSN:1559-6095
Publisher:Cold Spring Harbor Laboratory Press
Volume:2006
Number:6
Date:1 November 2006
Official Publication:https://doi.org/10.1101/pdb.prot4645
PubMed:View item in PubMed

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