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| Item Type: | Article |
|---|---|
| Title: | Signal requirements for growth and differentiation of activated murine B lymphocytes |
| Creators Name: | Müller, W., Kühn, R., Goldmann, W., Tesch, H., Smith, F.I., Radbruch, A. and Rajewsky, K. |
| Abstract: | Mouse B lymphocytes were stimulated at high cell concentrations with goat anti-IgM antibodies, which leads to the induction of B cell proliferation without the addition of any growth factors. After 48 hr, blast cells were purified and cultured at low cell concentrations. Proliferation and differentiation of purified B lymphocyte blasts is then dependent on the addition of either mitogens (e.g., LPS) or certain lymphokines derived from activated T cells or macrophages. One such lymphokine was isolated from supernatants of various activated T cells and characterized by gel filtration as a material with an apparent m.w. of 40,000 to 50,000, similar to BCGF II. It supports the proliferation of the B cell blasts and induces their differentiation into plaque-forming cells. Lymphokines such as BCGF I, interleukin 2, and BCDF gamma could neither maintain growth nor induce differentiation of B lymphocytes preactivated by goat anti-IgM. |
| Keywords: | B-Lymphocytes, Cell Differentiation, Cell Division, Gel Chromatography, Growth Substances, High Pressure Liquid Chromatography, Interleukin-4, Lymphocyte Activation, Lymphokines, Macrophages, T-Lymphocytes, Animals, Inbred BALB C Mice, Inbred C57BL Mice, Mice |
| Source: | Journal of Immunology |
| ISSN: | 0022-1767 |
| Publisher: | American Association of Immunologists |
| Volume: | 135 |
| Number: | 2 |
| Page Range: | 1213-1219 |
| Date: | August 1985 |
| Official Publication: | https://doi.org/10.4049/jimmunol.135.2.1213 |
| PubMed: | View item in PubMed |
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