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| Item Type: | Article |
|---|---|
| Title: | Rapid generation of inducible mouse mutants |
| Creators Name: | Seibler, J., Zevnik, B., Küter-Luks, B., Andreas, S., Kern, H., Hennek, T., Rode, A., Heimann, C., Faust, N., Kauselmann, G., Schoor, M., Jaenisch, R., Rajewsky, K., Kühn, R. and Schwenk, F. |
| Abstract: | We have generated an optimized inducible recombination system for conditional gene targeting based on a Cre recombinase-steroid receptor fusion. This configuration allows efficient Cre-mediated recombination in most organs of the mouse upon induction, without detectable background activity. An ES cell line, was established that carries the inducible recombinase and a loxP-flanked lacZ reporter gene. Out of this line, completely ES cell-derived mice were efficiently produced through tetraploid blastocyst complementation, without the requirement of mouse breeding. Our findings provide a new concept allowing the generation of inducible mouse mutants within 6 months, as compared to 14 months using the current protocol. |
| Keywords: | Cell Line, Gene Expression, Genetic Engineering, Genetic Recombination, Integrases, Knockout Mice, Mammalian Embryo, Recombinant Fusion Proteins, Steroid Receptors, Transgenic Mice, Viral Proteins, Animals, Mice |
| Source: | Nucleic Acids Research |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Volume: | 31 |
| Number: | 4 |
| Page Range: | e12 |
| Date: | 15 February 2003 |
| Official Publication: | https://doi.org/10.1093/nar/gng012 |
| PubMed: | View item in PubMed |
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