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Concerted down-regulation of immune-system related genes predicts metastasis in colorectal carcinoma

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Item Type:Article
Title:Concerted down-regulation of immune-system related genes predicts metastasis in colorectal carcinoma
Creators Name:Fehlker, M. and Huska, M.R. and Joens, T. and Andrade-Navarro, M.A. and Kemmner, W.
Abstract:BACKGROUND: This study aimed at the identification of prognostic gene expression markers in early primary colorectal carcinomas without metastasis at the time point of surgery by analyzing genome-wide gene expression profiles using oligonucleotide microarrays. METHODS: Cryo-conserved tumor specimens from 45 patients with early colorectal cancers were examined, with the majority of them being UICC stage II or earlier and with a follow-up time of 41-115 months. Gene expression profiling was performed using Whole Human Genome 4x44K Oligonucleotide Microarrays. Validation of microarray data was performed on five of the genes in a smaller cohort. RESULTS: Using a novel algorithm based on the recursive application of support vector machines (SVMs), we selected a signature of 44 probes that discriminated between patients developing later metastasis and patients with a good prognosis. Interestingly, almost half of the genes was related to the patients' immune response and showed reduced expression in the metastatic cases. CONCLUSIONS: Whereas up to now gene signatures containing genes with various biological functions have been described for prediction of metastasis in CRC, in this study metastasis could be well predicted by a set of gene expression markers consisting exclusively of genes related to the MHC class II complex involved in immune response. Thus, our data emphasize that the proper function of a comprehensive network of immune response genes is of vital importance for the survival of colorectal cancer patients.
Keywords:Early Colorectal Cancer, Metastasis, Computational Marker Analysis, Immune System, Gene Expression Profiling
Source:BMC Cancer
ISSN:1471-2407
Publisher:BioMed Central (U.K.)
Volume:14
Number:1
Page Range:64
Date:5 February 2014
Official Publication:https://doi.org/10.1186/1471-2407-14-64
PubMed:View item in PubMed

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