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Energy demand of synaptic transmission at the hippocampal Schaffer-collateral synapse

Item Type:Article
Title:Energy demand of synaptic transmission at the hippocampal Schaffer-collateral synapse
Creators Name:Liotta, A. and Roesner, J. and Huchzermeyer, C. and Wojtowicz, A. and Kann, O. and Schmitz, D. and Heinemann, U. and Kovacs, R.
Abstract:Neuroenergetic models of synaptic transmission predicted that energy demand is highest for action potentials (APs) and postsynaptic ion fluxes, whereas the presynaptic contribution is rather small. Here, we addressed the question of energy consumption at Schaffer-collateral synapses. We monitored stimulus-induced changes in extracellular potassium, sodium, and calcium concentration while recording partial oxygen pressure (pO(2)) and NAD(P)H fluorescence. Blockade of postsynaptic receptors reduced ion fluxes as well as pO(2) and NAD(P)H transients by ∼50%. Additional blockade of transmitter release further reduced Na(+), K(+), and pO(2) transients by ∼30% without altering presynaptic APs, indicating considerable contribution of Ca(2+)-removal, transmitter and vesicle turnover to energy consumption.
Keywords:Action Potential, Brain Slice, Electrophysiology, Energy Metabolism, Evoked Potentials, Hippocampus, Animals, Rats, Wistar Rats
Source:Journal of Cerebral Blood Flow and Metabolism
ISSN:0271-678X
Publisher:Nature Publishing Group (U.S.A.)
Volume:32
Number:11
Page Range:2076-2083
Date:November 2012
Official Publication:https://doi.org/10.1038/jcbfm.2012.116
PubMed:View item in PubMed

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