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JAK2 V617F allele burden quantified by real time quantitative polymerase chain reaction and competitive polymerase chain reaction in patients with chronic myeloproliferative neoplasia

Item Type:Article
Title:JAK2 V617F allele burden quantified by real time quantitative polymerase chain reaction and competitive polymerase chain reaction in patients with chronic myeloproliferative neoplasia
Creators Name:Kaeda, J. and Bonamino, M. and Ayres-Silva, J. and Solza, C. and Ringel, F. and Blau, O. and Daumas, A. and Oberender, C. and Doerken, B. and Le Coutre, P. and Zalcberg, I.
Abstract:Assessing the clinical significance of JAK2 V617F mutant allele burden is complicated by a myriad of techniques reported to detect and quantify the mutation. As a consequence, the level of sensitivity and how the data is reported vary. Harmonization of well-defined molecular studies would permit evaluation of the clinical significance of measuring allele burden and rapid determination of the efficacy of novel agents for the treatment of chronic myeloproliferative neoplasia via multicenter clinical trials, at the subclinical level. Here we report a comparison between the widely available TaqMan quantitative real time polymerase chain reaction (Q-PCR) and competitive PCR (C-PCR) assays. We found that the tumor load was invariably greater when measured by C-PCR compared to that recorded by Q-PCR. Furthermore, none of the samples converted from undetectable to detectable when the enriched granulocyte (GR) fraction was tested. While a difference in the V617F allele levels was detected between GR fraction and whole blood, this was not statistically significant.
Keywords:CMPN, JAK2, V617F, Quantification
Source:Leukemia & Lymphoma
ISSN:1042-8194
Publisher:Informa Healthcare (U.K.)
Volume:55
Number:1
Page Range:128-135
Date:January 2014
Official Publication:https://doi.org/10.3109/10428194.2013.797085
PubMed:View item in PubMed

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