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MicroRNA-143 down-regulates Hexokinase 2 in colon cancer cells

Item Type:Article
Title:MicroRNA-143 down-regulates Hexokinase 2 in colon cancer cells
Creators Name:Gregersen, L.H. and Jacobsen, A. and Frankel, L.B. and Wen, J. and Krogh, A. and Lund, A.H.
Abstract:BACKGROUND: MicroRNAs (miRNAs) are well recognized as gene regulators and have been implicated in the regulation of development as well as human diseases. miR-143 is located at a fragile site on chromosome 5 requently deleted in cancer, and has been reported to be down-regulated in several cancers including colon cancer. METHODS: To gain insight into the role of miR-143 in colon cancer, we used a microarray-based approach in combination with seed site enrichment analysis to identify miR-143 targets. RESULTS: As expected, transcripts down-regulated upon miR-143 overexpression had a significant enrichment of miR-143 seed sites in their 3'UTRs. Here we report the identification of Hexokinase 2 (HK2) as a direct target of miR-143. We show that re-introduction of miR-143 in the colon cancer cell line DLD-1 results in a decreased lactate secretion. CONCLUSION: We have identified and validated HK2 as a miR-143 target. Furthermore, our results indicate that miR-143 mediated down-regulation of HK2 affects glucose metabolism in colon cancer cells. We hypothesize that loss of miR-143-mediated repression of HK2 can promote glucose metabolism in cancer cells, contributing to the shift towards aerobic glycolysis observed in many tumors.
Keywords:miR-143, Colon Cancer, Hexokinase 2, Glycolysis
Source:BMC Cancer
ISSN:1471-2407
Publisher:BioMed Central (U.K.)
Volume:12
Page Range:232
Date:12 June 2012
Official Publication:https://doi.org/10.1186/1471-2407-12-232
PubMed:View item in PubMed

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