![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Tools
Tools
| Item Type: | Article |
|---|---|
| Title: | Human bone marrow as a source to generate CMV-specific CD4+ T cells with multifunctional capacity |
| Creators Name: | Na, I.K., Letsch, A., Guerreiro, M., Bauer, S., Noack, I., Geginat, J., Reinke, P., Loesch, M., Kienapfel, H., Thiel, E., Volk, H.D. and Scheibenbogen, C. |
| Abstract: | The bone marrow (BM) is an important compartment for T cell memory. In cytomegalovirus (CMV)-seropositive individuals peripheral blood (PB) CMV-specific T cells constitute a large fraction of PB T cells but are mostly differentiated effector/effector memory T cells with limited survival and proliferative potential. In this study, we performed a comprehensive analysis of the CMV-specific T cell response in BM studying both CD4+ and CD8+ T cell responses against overlapping peptide pools of the CMV proteins pp65 and immediate early protein-1. CMV-specific T cell responses were characterized ex vivo and after in vitro expansion of paired PB/BM samples by multiparameter flow cytometry determining surface phenotype, cytokine profile, and cytotoxic capability. Comparable frequencies of CMV-specific T cells were found in un-manipulated PB and BM. Both total CD4+ and CD8+ T cells could be more rapidly expanded from BM. Expanded BM T cells contained significantly higher frequencies of CMV-specific CD4+ T cells than PB. Furthermore, higher frequencies of specific CD4+ T cells from BM were multifunctional, characterized by simultaneous production of interferon-gamma, tumor necrosis factor, and interleukin-2. Use of BM may thus facilitate more rapid generation of adoptive T cells with enhanced functionality. |
| Keywords: | Multifunctional T Cells, CD4, CMV |
| Source: | Journal of Immunotherapy |
| ISSN: | 1524-9557 |
| Publisher: | Lippincott Williams & Wilkins |
| Volume: | 32 |
| Number: | 9 |
| Page Range: | 907-913 |
| Date: | November 2009 |
| Official Publication: | https://doi.org/10.1097/CJI.0b013e3181b7be60 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
