The Chriz-Z4 complex recruits JIL-1 to polytene chromosomes, a requirement for interband-specific phosphorylation of H3S10

Item Type:	Article
Title:	The Chriz-Z4 complex recruits JIL-1 to polytene chromosomes, a requirement for interband-specific phosphorylation of H3S10
Creators Name:	Gan, M. and Moebus, S. and Eggert, H. and Saumweber, H.
Abstract:	The conserved band-interband pattern is thought to reflect the looped-domain organization of insect polytene chromosomes. Previously, we have shown that the chromodomain protein Chriz and the zinc-finger protein Z4 are essentially required for the maintenance of polytene chromosome structure. Here we show that both proteins form a complex that recruits the JIL-1 kinase to polytene chromosomes, enabling local H3S10 phosphorylation of interband nucleosomal histones. Interband targeting domains were identified at the N-terminal regions of Chriz and Z4, and our data suggest partial cooperation of the complex with the BEAF boundary element protein in polytene and diploid cells. Reducing the core component Chriz by RNAi results in destabilization of the complex and a strong reduction of interband-specific histone H3S10 phosphorylation.
Keywords:	ChIP, Chromodomain, Immunoprecipitation, Zinc-Finger, Animals, Drosophila
Source:	Journal of Biosciences
ISSN:	0250-5991
Publisher:	Indian Academy of Sciences
Volume:	36
Number:	3
Page Range:	425-438
Date:	August 2011
Official Publication:	https://doi.org/10.1007/s12038-011-9089-y
PubMed:	View item in PubMed

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