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Degradation of inositol 1,3,4,5-tetrakisphosphates by porcine brain cytosol yields inositol 1,3,4-trisphosphate and inositol 1,4,5-trisphosphate

Item Type:Article
Title:Degradation of inositol 1,3,4,5-tetrakisphosphates by porcine brain cytosol yields inositol 1,3,4-trisphosphate and inositol 1,4,5-trisphosphate
Creators Name:Hoeer, D. and Kwiatkowski, A. and Seib, C. and Rosenthal, W. and Schultz, G. and Oberdisse, E.
Abstract:Inositol 1,3,4,5-tetrakisphosphates (Ins(1,3,4,5)P4), 32P-labelled in positions 4 and 5 were prepared enzymatically, using [4-32P]-phosphatidylinositol 4-phosphate (PtdInsP) and [5-32P]phosphatidylinositol 4,5-bisphosphate (PtdInsP2) as substrates, respectively. Degradation studies of Ins(1,3,4,5)P4, using an enriched phosphatase preparation from porcine brain cytosol, led to the formation of two inositol trisphosphate isomers which were identified as inositol 1,3,4-trisphosphate (Ins(1,3,4)P3) and inositol 1,4,5-trisphosphate (Ins(1,4,5)P3). This novel degradation pathway of Ins(1,3,4,5)P4 to Ins(1,4,5)P3 provides an additional source for the generation of Ins(1,4,5)P3, involving a 3-phosphatase.
Keywords:Brain, Cytosol, Inositol 1,4,5-Trisphosphate, Inositol Phosphates, Isomerism, Osmolar Concentration, Sugar Phosphates, Time Factors, Animals, Swine
Source:Biochemical and Biophysical Research Communications
ISSN:0006-291X
Publisher:Academic Press (U.S.A.)
Volume:154
Number:2
Page Range:668-675
Date:29 July 1988
Official Publication:https://doi.org/10.1016/0006-291X(88)90191-X
PubMed:View item in PubMed

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