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Heterogeneity of three electrophoretically distinct Go alpha-subunits in mammalian brain

Item Type:Article
Title:Heterogeneity of three electrophoretically distinct Go alpha-subunits in mammalian brain
Creators Name:Spicher, K. and Nuernberg, B. and Jaeger, B. and Rosenthal, W. and Schultz, G.
Abstract:So far three splice variants of the alpha o-gene coding for two alpha o proteins have been identified by molecular cloning, and the corresponding proteins purified. In the present study subtype-specific peptide antibodies revealed the existence of an electrophoretically distinct third form of alpha o in mammalian brain membranes which migrates more slowly on SDS-PAGE and shows a more acidic pI value than the other alpha o-subunits. Each of the three alpha o-subunits is detected as two isoforms when enriched from brain membranes. Rodent alpha o-subunits differ from non-rodent species in their electrophoretic mobilities. The results suggest that (i) there may exist a novel alpha o-subunit which reacts with an alpha o1-subunit-specific antibody, (ii) each alpha o-subunit may exist in more than one co- or posttranslationally modified isoform in brain membranes, and (iii) differences between alpha o-subunits from different species exist which are detectable by gel electrophoretic methods.
Keywords:G-protein, Go, 2-Dimensional-gel electrophoresis, Antipeptide antibody, Animals, Cattle, Rabbits, Rats, Swine, Mice
Source:FEBS Letters
ISSN:0014-5793
Publisher:Elsevier (The Netherlands)
Volume:307
Number:2
Page Range:215-218
Date:28 July 1992
Official Publication:https://doi.org/10.1016/0014-5793(92)80770-H
PubMed:View item in PubMed

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